ZIF-8 as a promising drug delivery system for benznidazole: development, characterization, in vitro dialysis release and cytotoxicity.

Autor: de Moura Ferraz LR; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil. prof.raphaelferraz@gmail.com., Tabosa AÉGA; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil., da Silva Nascimento DDS; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil., Ferreira AS; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil., de Albuquerque Wanderley Sales V; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil., Silva JYR; Laboratório de Terras Raras, Departamento de Química Fundamental, Federal University of Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE, 50740-560, Brazil., Júnior SA; Laboratório de Terras Raras, Departamento de Química Fundamental, Federal University of Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE, 50740-560, Brazil., Rolim LA; Central de Análise de Fármacos, Medicamentos e Alimentos, Federal University of Vale Do São Francisco, Av. José de Sá Maniçoba, s/n, Centro, Petrolina, PE, 56304-917, Brazil., de Souza Pereira JJ; Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, Brazil., Rolim-Neto PJ; Laboratório de Tecnologia Dos Medicamentos, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Arthur de Sá, s/n, Cidade Universitária, Recife, PE, 50740-521, Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Oct 08; Vol. 10 (1), pp. 16815. Date of Electronic Publication: 2020 Oct 08.
DOI: 10.1038/s41598-020-73848-w
Abstrakt: Chagas disease (CD), caused by the flagellate protozoan Trypanosoma cruzi, is one of the major public health problems in developing countries. Benznidazole (BNZ) is the only drug available for CD treatment in most countries, however, it presents high toxicity and low bioavailability. To address these problems this study used Zeolitic Imidazolate Framework-8 (ZIF-8), which has garnered considerable attention due to its potential applications, enabling the controlled delivery of drugs. The present work developed and characterized a BNZ@ZIF-8 system, and the modulation of BNZ release from the ZIF-8 framework was evaluated through the in vitro dialysis release method under sink conditions at different pH values. Moreover, the in vitro evaluation of cell viability and cytotoxicity by MTT assay were also performed. The dissolution studies corroborated that a pH sensitive Drug Delivery System capable of vectorizing the release of BNZ was developed, may leading to the improvement in the bioavailability of BNZ. The MTT assay showed that no statistically significant toxic effects occurred in the developed system, nor significant effects on cell viability.
Databáze: MEDLINE
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