The role of Rho1 gene in the cell wall integrity and polysaccharides biosynthesis of the edible mushroom Grifola frondosa.

Autor: Zan XY; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China., Zhu HA; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China., Jiang LH; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China., Liang YY; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China., Sun WJ; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing 334221, PR China., Tao TL; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China., Cui FJ; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing 334221, PR China. Electronic address: fengjiecui@ujs.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 15; Vol. 165 (Pt A), pp. 1593-1603. Date of Electronic Publication: 2020 Oct 05.
DOI: 10.1016/j.ijbiomac.2020.09.239
Abstrakt: Grifola frondosa polysaccharides, especially β-glucans, showed the significant antitumor, hypoglycemic, and immune-stimulating activities. In the present study, a predominant regulatory subunit gfRho1p of β-1,3-glucan synthase in G. frondosa was identified with a molecular weight of 20.79 kDa and coded by a putative 648-bp small GTPase gene gfRho1. By constructing mutants of RNA interference and over-expression gfRho1, the roles of gfRho1 in the growth, cell wall integrity and polysaccharide biosynthesis were well investigated. The results revealed that defects of gfRho1 slowed mycelial growth rate by 22% to 33%, reduced mycelial polysaccharide and exo-polysaccharide yields by 4% to 7%, increased sensitivity to cell wall stress, and down-regulated gene transcriptions related to PKC-MAPK signaling pathway in cell wall integrity. Over-expression of gfRho1 improved mycelial growth rate and polysaccharide production of G. frondosa. Our study supports that gfRho1 is an essential regulator for polysaccharide biosynthesis, cell growth, cell wall integrity and stress response in G. frondosa.
Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: