The inhibitory effect of the deubiquitinase cylindromatosis (CYLD) on inflammatory responses in human gingival fibroblasts.

Autor: Fu YW; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.; Department of Stomatology, The First People's Hospital of Lianyungang, Lianyungang, China., Li L; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China., Wang XQ; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China., Zhou Y; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China., Zhu LF; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China., Mei YM; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China., Xu Y; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.; Department of Periodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2021 Sep; Vol. 27 (6), pp. 1487-1497. Date of Electronic Publication: 2020 Oct 22.
DOI: 10.1111/odi.13672
Abstrakt: Objective: Experiments were performed to evaluate CYLD expression in human gingival tissue samples and to examine the effects of CYLD on inflammatory responses in lipopolysaccharide (LPS)- or TNF-α-stimulated human gingival fibroblasts (HGFs).
Methods: Immunohistochemistry for CYLD and p65 expression was performed with healthy and inflamed gingival tissue samples. siRNA was used to knock down the expression of CYLD in HGFs. Upon LPS or TNF-α stimulation, NF-κB activation was detected in control and CYLD-knockdown HGFs. RT-PCR was applied to determine gene expression. Western blot analyses were employed to assess protein expression. Immunofluorescence staining was carried out to evaluate the nuclear translocation of p65.
Results: Immunohistochemical staining showed the expression of CYLD in human gingival tissues. In addition, CYLD protein expression was reduced in inflamed gingival tissue samples compared with healthy tissue samples. CYLD knockdown greatly enhanced the mRNA expression of proinflammatory cytokines in LPS- or TNF-α-stimulated HGFs. Furthermore, knocking down CYLD expression increased LPS-stimulated NF-κB activation in HGFs. Unexpectedly, CYLD knockdown did not affect TNF-α-induced NF-κB activation.
Conclusions: Our results suggest that CYLD participates in periodontal inflammatory responses by negatively regulating LPS-induced NF-κB signalling.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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