Precision 3D-Printed Cell Scaffolds Mimicking Native Tissue Composition and Mechanics.

Autor: Erben A; Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, Munich, 80533, Germany.; Heinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, Campus Klinikum rechts der Isar, Technical University of Munich, Einsteinstraße 25, Munich, 81675, Germany.; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany., Hörning M; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, Stuttgart, 70569, Germany., Hartmann B; Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, Munich, 80533, Germany.; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany., Becke T; Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, Munich, 80533, Germany.; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany., Eisler SA; Stuttgart Research Center Systems Biology, University of Stuttgart, Nobelstr. 15, Stuttgart, 70569, Germany., Southan A; Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobelstr. 12, Stuttgart, 70569, Germany., Cranz S; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, Munich, 81377, Germany.; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, Max-Lebsche-Platz 31, Munich, 81377, Germany., Hayden O; Heinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, Campus Klinikum rechts der Isar, Technical University of Munich, Einsteinstraße 25, Munich, 81675, Germany., Kneidinger N; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, Munich, 81377, Germany.; Department of Internal Medicine V, Ludwig-Maximillians-University Munich, Marchioninistr. 15, Munich, 81377, Germany., Königshoff M; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, Munich, 81377, Germany.; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, Max-Lebsche-Platz 31, Munich, 81377, Germany.; University of Colorado, Department of Pulmonary Sciences and Critical Care Medicine, 13001 E. 17th Pl., Aurora, CO, 80045, USA., Lindner M; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, Munich, 81377, Germany.; University Department of Visceral and Thoracic Surgery Salzburg, Paracelsus Medical University, Müllner Hauptstraße 48, Salzburg, A-5020, Austria., Tovar GEM; Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobelstr. 12, Stuttgart, 70569, Germany., Burgstaller G; Institute of Lung Biology and Disease and Comprehensive Pneumology Center with the CPC-M bioArchive, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, Munich, 81377, Germany.; Institute of Lung Biology and Disease (ILBD), Helmholtz Zentrum München, Max-Lebsche-Platz 31, Munich, 81377, Germany., Clausen-Schaumann H; Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, Munich, 80533, Germany.; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany., Sudhop S; Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, Munich, 80533, Germany.; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany., Heymann M; Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, Munich, 80539, Germany.; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, Stuttgart, 70569, Germany.; Department of Cellular and Molecular Biophysics, MPI of Biochemistry Martinsried, Am Klopferspitz 18, Planegg, 82152, Germany.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2020 Dec; Vol. 9 (24), pp. e2000918. Date of Electronic Publication: 2020 Oct 07.
DOI: 10.1002/adhm.202000918
Abstrakt: Cellular dynamics are modeled by the 3D architecture and mechanics of the extracellular matrix (ECM) and vice versa. These bidirectional cell-ECM interactions are the basis for all vital tissues, many of which have been investigated in 2D environments over the last decades. Experimental approaches to mimic in vivo cell niches in 3D with the highest biological conformity and resolution can enable new insights into these cell-ECM interactions including proliferation, differentiation, migration, and invasion assays. Here, two-photon stereolithography is adopted to print up to mm-sized high-precision 3D cell scaffolds at micrometer resolution with defined mechanical properties from protein-based resins, such as bovine serum albumin or gelatin methacryloyl. By modifying the manufacturing process including two-pass printing or post-print crosslinking, high precision scaffolds with varying Young's moduli ranging from 7-300 kPa are printed and quantified through atomic force microscopy. The impact of varying scaffold topographies on the dynamics of colonizing cells is observed using mouse myoblast cells and a 3D-lung microtissue replica colonized with primary human lung fibroblast. This approach will allow for a systematic investigation of single-cell and tissue dynamics in response to defined mechanical and bio-molecular cues and is ultimately scalable to full organs.
(© 2020 The Authors. Published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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