Streptococcus agalactiae : Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women.

Autor:	Santana FAF; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., de Oliveira TVL; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Filho MBS; Laboratório Oliveira Ltda, LABO, Vitória da Conquista 45020740, Bahia, Brazil., da Silva LSC; Campus Soane Nazaré de Andrade, Universidade Estadual de Santa Cruz, Ilhéus 45662900, Bahia, Brazil., de Brito BB; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., de Melo FF; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil. freiremelo@yahoo.com.br., Souza CL; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Marques LM; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Oliveira MV; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil.
Jazyk:	angličtina
Zdroj:	World journal of clinical cases [World J Clin Cases] 2020 Sep 26; Vol. 8 (18), pp. 3988-3998.
DOI:	10.12998/wjcc.v8.i18.3988
Abstrakt:	Background: Group B Streptococcus (GBS) is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in newborns. Aim: To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista, in Bahia State, Brazil. Methods: From January 2017 to February 2018, vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective broth. Confirmatory identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive colonies. Then, disk diffusion antibiograms were performed in GBS-positive samples, and the detection of the resistance genes erm B, erm TR, mef A, and lin B in the clindamycin and/or erythromycin-resistant samples was carried out. Results: Thirty-two samples (17.2%) were GBS-positive. The culture in chromogenic agar after sample incubation in selective broth was the most sensitive method (96.9%) for GBS detection. All isolates were susceptible to penicillin, ampicillin, cefotaxime, and vancomycin. Clindamycin resistance was observed in 6 samples (18.8%), while 8 samples (25%) were erythromycin-resistant. All erythromycin and/or clindamycin-resistant GBS strains had negative D-tests. Two strains (25%) presented an M phenotype and 6 isolates (75%) presented a cMLSB phenotype. The erm B gene was identified in 4 samples (44.4%), the mef A gene was also found in 4 samples (44.4%), the erm TR gene was identified in 1 isolate (11.1%), and the lin B gene was not found in any isolate. Conclusion: This study evidenced that the screening for SGB can be performed by means of various methods, including chromogenic media, and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided. Competing Interests: Conflict-of-interest statement: There is no conflict of interest associated with any of the senior author or other coauthors contributed their efforts in this manuscript. (©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.)
Databáze:	MEDLINE
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