Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens.

Autor: van Beek LF; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands.; Radboud Center for Infectious Diseases , Nijmegen, The Netherlands., Surmann K; Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , Greifswald, Germany., van den Berg van Saparoea HB; Abera Bioscience AB , Solna, Sweden., Houben D; Abera Bioscience AB , Solna, Sweden., Jong WSP; Abera Bioscience AB , Solna, Sweden., Hentschker C; Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , Greifswald, Germany., Ederveen THA; Center for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center , Nijmegen, The Netherlands., Mitsi E; Liverpool School of Tropical medicine, Respiratory Infection Group , Liverpool, United Kingdom of Great Britain and Northern Ireland., Ferreira DM; Liverpool School of Tropical medicine, Respiratory Infection Group , Liverpool, United Kingdom of Great Britain and Northern Ireland., van Opzeeland F; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands.; Radboud Center for Infectious Diseases , Nijmegen, The Netherlands., van der Gaast-de Jongh CE; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands.; Radboud Center for Infectious Diseases , Nijmegen, The Netherlands., Joosten I; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands., Völker U; Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , Greifswald, Germany., Schmidt F; Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , Greifswald, Germany.; Proteomics Core, Weill Cornell Medicine-Qatar , Doha, Qatar., Luirink J; Abera Bioscience AB , Solna, Sweden.; Department of Molecular Microbiology, Faculty of Science, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam , Amsterdam, The Netherlands., Diavatopoulos DA; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands.; Radboud Center for Infectious Diseases , Nijmegen, The Netherlands., de Jonge MI; Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences , Nijmegen, The Netherlands.; Radboud Center for Infectious Diseases , Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: Virulence [Virulence] 2020 Dec; Vol. 11 (1), pp. 1310-1328.
DOI: 10.1080/21505594.2020.1825908
Abstrakt: Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae . Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.
Databáze: MEDLINE