Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts.

Autor: Oubihi A; Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, Morocco., Hosni H; Pharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco., Nounah I; Laboratory of Chemistry and Organic and Bioorganic Synthesis, Faculty of Science, Geophysics, Natural Patrimony and Green Chemistry (GEOPAC) Research Center, University Mohammed V, Rabat, Morocco., Ettouil A; Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, Morocco., Harhar H; Laboratory of Nanotechnology, Materials and Environment, Department of Chemistry, Faculty of Science, Mohammed V University in Rabat, BP. 1014, Rabat, Morocco., Alaoui K; Pharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco., Ouhssine M; Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, Morocco., Guessous Z; Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, Morocco.
Jazyk: angličtina
Zdroj: Biochemistry research international [Biochem Res Int] 2020 Sep 18; Vol. 2020, pp. 8823209. Date of Electronic Publication: 2020 Sep 18 (Print Publication: 2020).
DOI: 10.1155/2020/8823209
Abstrakt: Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Asmaa Oubihi et al.)
Databáze: MEDLINE
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