| Autor: |
Henker LC; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil. luanchenker@gmail.com., Bandinelli MB; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., de Andrade CP; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., Bianchi MV; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., Sonne L; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., Driemeier D; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., Soares JF; Laboratório de Protozoologia e Rickettsiosis Vetoriais, UFRGS, Avenida Bento Gonçalves 9090, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil., Pavarini SP; Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Prédio 42.505, Bairro Agronomia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil. |
| Abstrakt: |
Equine protozoal myeloencephalitis (EPM) is an important neurologic disease of horses in the American continent caused by Sarcocystis neurona and Neospora hughesi infection. This study describes the pathological, immunohistochemical, and molecular findings of fatal cases of EPM in southern Brazil. A review was performed on a total of 13 cases compatible with EPM, which were diagnosed by postmortem examination in the period of 2010-2017. Epidemiological information was obtained from necropsy reports. Gross and histological lesions were characterized, and cases were subjected to immunohistochemistry anti-Sarcocystis neurona, Toxoplasma gondii, and Neospora spp. Molecular search was performed using ITS-1 gene PCRs. Microscopic lesions were multifocal in all cases, and more frequently observed in the spinal cord segments and in the rhombencephalon. Intralesional protozoans were histologically detected in five horses, while a positive immunostaining for S. neurona was observed in eleven cases (11/13). Through molecular techniques, six positive cases for the ITS-1 gene were detected, and obtained sequences presented highest similarity with S. neurona. EPM due to S. neurona infection represents an important neurologic disease of horses in Brazil and this disease should be considered as a main differential diagnosis in horses presenting neurologic signs. |
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