Chemical characterization, antioxidant, antibacterial and enzyme inhibitory properties of Canthium coromandelicum, a valuable source for bioactive compounds.

Autor: Amalraj S; Department of Botany, A. Veeriya Vandayar Memorial Sri Pushpam College (Autonomous), Affiliated to Bharathidasan University, Poondi, Thanjavur, 613 503, India., Krupa J; Department of Botany, A. Veeriya Vandayar Memorial Sri Pushpam College (Autonomous), Affiliated to Bharathidasan University, Poondi, Thanjavur, 613 503, India., Sriramavaratharajan V; School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, 613 401, India., Mariyammal V; Department of Botany, A. Veeriya Vandayar Memorial Sri Pushpam College (Autonomous), Affiliated to Bharathidasan University, Poondi, Thanjavur, 613 503, India., Murugan R; Centre for Research and Postgraduate Studies in Botany, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, 626 124, India., Ayyanar M; Department of Botany, A. Veeriya Vandayar Memorial Sri Pushpam College (Autonomous), Affiliated to Bharathidasan University, Poondi, Thanjavur, 613 503, India. Electronic address: asmayyanar@yahoo.com.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2021 Jan 05; Vol. 192, pp. 113620. Date of Electronic Publication: 2020 Sep 15.
DOI: 10.1016/j.jpba.2020.113620
Abstrakt: The present study aimed at chemical characterization of Canthium coromandelicum leaf extracts (CCLE) and their in vitro pharmacological (antioxidant, enzyme inhibitory, and antibacterial) activities. Chemical characterization includes chemical profile of six extracts of CC by Gas chromatography - Mass spectrometry (GC-MS) analysis and total phenolics and flavonoids by spectrophotometric methods. Antioxidant activity was determined using eight assays. Enzymatic inhibitory property was evaluated by α-amylase and α-glucosidase inhibitory assays and antibacterial activity was studied against 10 pathogenic bacteria by agar disc diffusion method. GC-MS analysis enabled the identification of 65 compounds with palmitic acid, n-pentacosane, cycloartenol, linoleic acid, squalene, γ-sitosterol, nonacosane and α-tocopherol as major constituents of CCLE. Highest amount of total phenolics (58.03 mg GAE/g extract) and flavonoids (44.40 QE/g) was present in hydroalcoholic extract. Hydroalcoholic, methanolic and aqueous extracts showed significant free radical scavenging abilities and positive correlation was detected between antioxidant assays with recorded phenolics and flavonoids. Hydroalcoholic and methanolic extracts exhibited significant α-amylase (IC 50 of 44.25 μg/mL) and α-glucosidase inhibitory activities (IC 50 of 30.82 μg/mL) respectively.Methanolic and hydroalcoholic extracts at 750 μg/mL showed maximum antibacterial activity against S. typhi and S. flexneri respectively. Also, significant correlation was found between V. cholerae and R. equi as well as V. cholerae and S. epidermis. To conclude, C. coromandelicum could be considered as a natural antioxidant and potential source for therapeutic applications. However, widespread study is necessary to screen the role of recorded phytochemicals through in vivo studies to support its use in traditional medicine.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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