CD38 downregulation modulates NAD + and NADP(H) levels in thermogenic adipose tissues.

Autor: Benzi A; DIMES-Section of Biochemistry, University of Genova, Italy., Sturla L; DIMES-Section of Biochemistry, University of Genova, Italy. Electronic address: laurasturla@unige.it., Heine M; Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Fischer AW; Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Spinelli S; DIMES-Section of Biochemistry, University of Genova, Italy., Magnone M; DIMES-Section of Biochemistry, University of Genova, Italy., Sociali G; DIMES-Section of Biochemistry, University of Genova, Italy., Parodi A; IRCCS Ospedale Policlinico San Martino, Genova, Italy., Fenoglio D; IRCCS Ospedale Policlinico San Martino, Genova, Italy; Department of Internal Medicine, University of Genova, Italy., Emionite L; Animal Facility, IRCCS Ospedale Policlinico San Martino, Largo Benzi 10, 16132 Genova, Italy., Koch-Nolte F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Mittrücker HW; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Guse AH; Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., De Flora A; DIMES-Section of Biochemistry, University of Genova, Italy., Zocchi E; DIMES-Section of Biochemistry, University of Genova, Italy., Heeren J; Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Bruzzone S; DIMES-Section of Biochemistry, University of Genova, Italy. Electronic address: santina.bruzzone@unige.it.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Molecular and cell biology of lipids [Biochim Biophys Acta Mol Cell Biol Lipids] 2021 Jan; Vol. 1866 (1), pp. 158819. Date of Electronic Publication: 2020 Sep 30.
DOI: 10.1016/j.bbalip.2020.158819
Abstrakt: Different strategies to boost NAD + levels are considered promising means to promote healthy aging and ameliorate dysfunctional metabolism. CD38 is a NAD + -dependent enzyme involved in the regulation of different cell functions. In the context of systemic energy metabolism, it has been demonstrated that brown adipocytes, the parenchymal cells of brown adipose tissue (BAT) as well as beige adipocytes that emerge in white adipose tissue (WAT) depots in response to catabolic conditions, are important to maintain metabolic homeostasis. In this study we aim to understand the functional relevance of CD38 for NAD + and energy metabolism in BAT and WAT, also using a CD38 -/- mouse model. During cold exposure, an increase in NAD + levels occurred in BAT of wild type mice, together with a marked downregulation of CD38, as detected at the mRNA and protein level. CD38 downregulation was observed also in WAT of cold-exposed mice, where it was accompanied by a strong increase in NADP(H) levels. Accordingly, NAD kinase and glucose-6-phosphate dehydrogenase activities were enhanced in WAT (but not in BAT). Increased NAD + levels were observed in BAT/WAT from CD38 -/- compared with wild type mice, in line with CD38 being a major NAD + -consumer in AT. CD38 -/- mice kept at 6 °C had higher levels of Ucp1 and Pgc-1α in BAT and WAT, and increased levels of phosphorylated hormone-sensitive lipase in BAT, compared with wild type mice. These results demonstrate that CD38, by modulating cellular NAD(P) + levels, is involved in the regulation of thermogenic responses in cold-activated BAT and WAT.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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