Molecular characterization of the first reported Aichivirus A in Australia.
| Autor: | Northill JA; Public Health Virology, Forensic and Scientific Services, Coopers Plains, QLD, Australia., Simmons RJ; Public Health Virology, Forensic and Scientific Services, Coopers Plains, QLD, Australia., Genge D; Public Health Virology, Forensic and Scientific Services, Coopers Plains, QLD, Australia., Moore FA; Public Health Virology, Forensic and Scientific Services, Coopers Plains, QLD, Australia. |
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| Jazyk: | angličtina |
| Zdroj: | Access microbiology [Access Microbiol] 2020 Feb 11; Vol. 2 (4), pp. acmi000099. Date of Electronic Publication: 2020 Feb 11 (Print Publication: 2020). |
| DOI: | 10.1099/acmi.0.000099 |
| Abstrakt: | A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect Aichivirus A (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitted for norovirus testing during May 2008 detected a further 23 AiV-A-positive samples from 18 individual patients. Genotyping using novel primers targeting the 3C-3D junction region identified AiV-A genotype B. Further sequencing of the VP1 region supported the 3C-3D result. All three assays proved useful to support foodborne outbreak investigations. This is the first report of AiV-A detection in Australia. Competing Interests: The authors declare that there are no conflicts of interest. (© 2020 The Authors.) |
| Databáze: | MEDLINE |
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