Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis.

Autor: Klempt P; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic., Brož P; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.; Department of Biology and Medical Genetics, Second Faculty of Medicine, Charles University and Motol University Hospital, V Úvalu 84, 150 06 Prague, Czech Republic., Kašný M; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic., Novotný A; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic., Kvapilová K; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.; Faculty of Science, Charles University, Albertov 6, 128 00 Prague, Czech Republic., Kvapil P; Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2020 Sep 29; Vol. 10 (10). Date of Electronic Publication: 2020 Sep 29.
DOI: 10.3390/diagnostics10100769
Abstrakt: Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at the global level Until today, thousands of SARS-CoV-2 genome sequences have been published at GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable for reliable variant analysis. Here we report on the comparison of three commercially available NGS library preparation kits. We discuss advantages and limitations from the perspective of required input sample quality and data quality for advanced SARS-CoV-2 genome analysis.
Databáze: MEDLINE
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