In utero human intestine harbors unique metabolome, including bacterial metabolites.

Autor:	Li Y; Department of Biostatistics and., Toothaker JM; Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Ben-Simon S; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel., Ozeri L; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel., Schweitzer R; Analytical Chemistry Laboratory, Tel-Hai College, Upper Galilee, Israel., McCourt BT; Department of Pediatrics, Yale University, New Haven, Connecticut, USA., McCourt CC; Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Werner L; Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat Gan, Israel.; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Snapper SB; Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, Massachusetts, USA., Shouval DS; Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat Gan, Israel.; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Khatib S; Analytical Chemistry Laboratory, Tel-Hai College, Upper Galilee, Israel.; Department of Natural Compounds and Analytical Chemistry, Migal Galilee Research Institute, Kiryat Shmona, Israel., Koren O; Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel., Agnihorti S; Department of Neurosurgery and., Tseng G; Department of Biostatistics and., Konnikova L; Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Pediatrics, Yale University, New Haven, Connecticut, USA.; Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Developmental Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Division of Reproductive Sciences and.; Program in Human and Translational Immunology, Yale University, New Haven, Connecticut, USA.
Jazyk:	angličtina
Zdroj:	JCI insight [JCI Insight] 2020 Nov 05; Vol. 5 (21). Date of Electronic Publication: 2020 Nov 05.
DOI:	10.1172/jci.insight.138751
Abstrakt:	Symbiotic microbial colonization through the establishment of the intestinal microbiome is critical to many intestinal functions, including nutrient metabolism, intestinal barrier integrity, and immune regulation. Recent studies suggest that education of intestinal immunity may be ongoing in utero. However, the drivers of this process are unknown. The microbiome and its byproducts are one potential source. Whether a fetal intestinal microbiome exists is controversial, and whether microbially derived metabolites are present in utero is unknown. Here, we aimed to determine whether bacterial DNA and microbially derived metabolites can be detected in second trimester human intestinal samples. Although we were unable to amplify bacterial DNA from fetal intestines, we report a fetal metabolomic intestinal profile with an abundance of bacterially derived and host-derived metabolites commonly produced in response to microbiota. Though we did not directly assess their source and function, we hypothesize that these microbial-associated metabolites either come from the maternal microbiome and are vertically transmitted to the fetus to prime the fetal immune system and prepare the gastrointestinal tract for postnatal microbial encounters or are produced locally by bacteria that were below our detection threshold. Competing Interests: ex
Databáze:	MEDLINE
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