Vitamin K2 Modulates Vitamin D-Induced Mechanical Properties of Human 3D Bone Spheroids In Vitro.
Autor: | Schröder M; Department of Biomaterials University of Oslo Oslo Norway., Riksen EA; Department of Biomaterials University of Oslo Oslo Norway., He J; Department of Structural Engineering, Faculty of Engineering Norwegian University of Science and Technology (NTNU) Trondheim Norway., Skallerud BH; Department of Structural Engineering, Faculty of Engineering Norwegian University of Science and Technology (NTNU) Trondheim Norway., Møller ME; Axial Vita AS Oslo Norway., Lian AM; Oral Research Laboratory, Institute for Clinical Dentistry University of Oslo Oslo Norway., Syversen U; Oral Research Laboratory, Institute for Clinical Dentistry University of Oslo Oslo Norway.; Department of Clinical and Molecular Medicine NTNU Trondheim Norway.; Department of Endocrinology, Clinic of Medicine, St. Olavs Hospital Trondheim University Hospital Trondheim Norway., Reseland JE; Department of Biomaterials University of Oslo Oslo Norway. |
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Jazyk: | angličtina |
Zdroj: | JBMR plus [JBMR Plus] 2020 Aug 03; Vol. 4 (9), pp. e10394. Date of Electronic Publication: 2020 Aug 03 (Print Publication: 2020). |
DOI: | 10.1002/jbm4.10394 |
Abstrakt: | Rotational culture promotes primary human osteoblasts (hOBs) to form three-dimensional (3D) multicellular spheroids with bone tissue-like structure without any scaffolding material. Cell-based bone models enable us to investigate the effect of different agents on the mechanical strength of bone. Given that low dietary intake of both vitamin D and K is negatively associated with fracture risk, we aimed to assess the effect of these vitamins in this system. Osteospheres of hOBs were generated with menaquinone-4 (MK-4; 10μM) and 25-hydroxyvitamin D (© 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.) |
Databáze: | MEDLINE |
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