H 2 O 2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum.

Autor: Amblard I; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France.; Sorbonne Université, Paris, France., Thauvin M; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France.; Sorbonne Université, Paris, France., Rampon C; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France.; Université de Paris, Faculty of Sciences, Paris, France., Queguiner I; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France., Pak VV; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, 117997, Russia.; Institute for Cardiovascular Physiology, Georg August University Göttingen, 37073, Göttingen, Germany., Belousov V; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, 117997, Russia.; Institute for Cardiovascular Physiology, Georg August University Göttingen, 37073, Göttingen, Germany.; Federal Center of Brain Research and Neurotechnologies, FMBA, Moscow, 117997, Russia., Prochiantz A; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France., Volovitch M; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France.; Department of Biology, École Normale Supérieure, PSL Research University, Paris, France., Joliot A; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France. alain.joliot@college-de-france.fr., Vriz S; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris, France. vriz@univ-paris-diderot.fr.; Université de Paris, Faculty of Sciences, Paris, France. vriz@univ-paris-diderot.fr.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2020 Sep 29; Vol. 3 (1), pp. 536. Date of Electronic Publication: 2020 Sep 29.
DOI: 10.1038/s42003-020-01268-7
Abstrakt: Although a physiological role for redox signaling is now clearly established, the processes sensitive to redox signaling remains to be identified. Ratiometric probes selective for H 2 O 2 have revealed its complex spatiotemporal dynamics during neural development and adult regeneration and perturbations of H 2 O 2 levels disturb cell plasticity and morphogenesis. Here we ask whether endogenous H 2 O 2 could participate in the patterning of the embryo. We find that perturbations of endogenous H 2 O 2 levels impact on the distribution of the Engrailed homeoprotein, a strong determinant of midbrain patterning. Engrailed 2 is secreted from cells with high H 2 O 2 levels and taken up by cells with low H 2 O 2 levels where it leads to increased H 2 O 2 production, steering the directional spread of the Engrailed gradient. These results illustrate the interplay between protein signaling pathways and metabolic processes during morphogenetic events.
Databáze: MEDLINE
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