Reduced graphene oxide membranes in ocular regenerative medicine.

Autor: Zambrano-Andazol I; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Vázquez N; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Chacón M; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Sánchez-Avila RM; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Persinal M; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Blanco C; Instituto de Ciencia y Tecnología del Carbono, INCAR-CSIC, Asturias, Spain., González Z; Instituto de Ciencia y Tecnología del Carbono, INCAR-CSIC, Asturias, Spain., Menéndez R; Instituto de Ciencia y Tecnología del Carbono, INCAR-CSIC, Asturias, Spain., Sierra M; Departamento de Biología Funcional, Área de Genética, Universidad de Oviedo, Asturias, Spain., Fernández-Vega Á; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Sánchez T; Unidad de Bioterio e Imagen Preclínica, Universidad de Oviedo, Asturias, Spain., Merayo-Lloves J; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain., Meana Á; Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, Asturias, Spain; Centro Comunitario de Sangre y Tejidos de Asturias, Asturias, Spain. CIBERER U714. Electronic address: meana@fio.as.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2020 Sep; Vol. 114, pp. 111075. Date of Electronic Publication: 2020 May 11.
DOI: 10.1016/j.msec.2020.111075
Abstrakt: Biological membranes are currently used in Ophthalmology in order to treat different ocular disorders. These membranes have different properties such as cellular biocompatibility and promoting wound healing. Moreover, intrinsic antimicrobial properties could also be desirable because it would allow their use reducing the risk of infections. Graphene and its derivatives are promising biomaterials that already proved their bactericidal effect. However, their clinical use is limited due to the controversial results regarding their toxicity. In this work, we have developed and characterized a reduced graphene oxide membrane (rGOM) for its use in ocular Regenerative Medicine, and studied its in vitro and in vivo biocompatibility and genotoxicity with different types of human ocular cells. We proved that rGOM allowed the growth of different ocular cells without inducing in vitro or in vivo cytotoxicity or genotoxicity in the short-term. These results indicate that rGOM may be a promising candidate in Regenerative Medicine for the treatment of different ocular pathologies.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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