Assessment of the Hemotek® system for the in vitro feeding of field-collected Culicoides imicola (Diptera: Ceratopogonidae) in South Africa.

Autor: de Beer CJ; Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Insect Pest Control Laboratory, Vienna, Austria.; Epidemiology, Vectors and Parasites, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria, South Africa., Boikanyo SNB; Epidemiology, Vectors and Parasites, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria, South Africa., Venter GJ; Epidemiology, Vectors and Parasites, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria, South Africa.; Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa.
Jazyk: angličtina
Zdroj: Medical and veterinary entomology [Med Vet Entomol] 2021 Jun; Vol. 35 (2), pp. 177-186. Date of Electronic Publication: 2020 Sep 29.
DOI: 10.1111/mve.12484
Abstrakt: The optimising and standardisation of in vitro blood feeding protocols for field-collected Culicoides species (Diptera: Ceratopogonidae) will be of essence for the comparison of the vector competencies of various populations of viruses of veterinary importance and the establishment of laboratory colonies of putative vector species. A custom-made feeding chamber to accommodate the small size of Culicoides imicola Kieffer was designed for the commercially available Hemotek® system and compared to existing membrane and cotton pledge feeding methods. High feeding rates coupled to higher mean blood meal volume than that of the existing OVI device indicated that the Hemotek system will be suitable for the feeding of field-collected Culicoides. The Hemotek system was subsequently used to identify factors that may affect feeding success in the laboratory. Evaluated factors were the source (host) and temperature of the blood meal, time of the day of feeding, the position of the blood reservoir in relation to the midges and exposure time to the blood. While only feeding orientation and the temperature of the blood source seems to significantly affect the feeding rate, all the factors did influence the volume of blood consumed.
(© 2020 The Royal Entomological Society.)
Databáze: MEDLINE
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