| Autor: |
Jung KW; Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup o56212, Republic of Korea., Lee KT; Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea., Bahn YS; Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea. ysbahn@yonsei.ac.kr. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of microbiology (Seoul, Korea) [J Microbiol] 2020 Oct; Vol. 58 (10), pp. 823-831. Date of Electronic Publication: 2020 Sep 29. |
| DOI: |
10.1007/s12275-020-0341-8 |
| Abstrakt: |
Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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