| Autor: |
Teh MY; School of Biological Sciences, Department of Molecular and Biomedical Science, Research Centre for Infectious Diseases, University of Adelaide, Adelaide, Australia., Furevi A; Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden., Widmalm G; Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden., Morona R; School of Biological Sciences, Department of Molecular and Biomedical Science, Research Centre for Infectious Diseases, University of Adelaide, Adelaide, Australia renato.morona@adelaide.edu.au. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of bacteriology [J Bacteriol] 2020 Nov 19; Vol. 202 (24). Date of Electronic Publication: 2020 Nov 19 (Print Publication: 2020). |
| DOI: |
10.1128/JB.00363-20 |
| Abstrakt: |
Shigella flexneri is a major causative agent of bacillary dysentery in developing countries, where serotype 2a 2 is the prevalent strain. To date, approximately 30 serotypes have been identified for S. flexneri , and the major contribution to the emergence of new serotypes is chemical modifications of the lipopolysaccharide (LPS) component O antigen (Oag). Glucosylation, O-acetylation, and phosphoethanolamine (PEtN) modifications increase the Oag diversity, providing benefits to S. flexneri LPS Oag acts as a primary receptor for bacteriophage Sf6, which infects only a limited range of S. flexneri serotypes (Y and X). It uses its tailspike protein (Sf6TSP) to establish initial interaction with LPS Oags that it then hydrolyzes. Currently, there is a lack of comprehensive study on the parent and serotype variant strains from the same genetic background and an understanding of the importance of LPS Oag O-acetylations. Therefore, a set of isogenic strains (based on S. flexneri 2457T [2a 2 ]) with deletions of different Oag modification genes ( oacB , oacD , and gtrII ) that resemble different naturally occurring serotype Y and 2a strains was created. The impacts of these Oag modifications on S. flexneri sensitivity to Sf6 and the pathogenesis-related properties were then compared. We found that Sf6TSP can hydrolyze serotype 2a LPS Oag, identified that 3/4-O-acetylation is essential for resistance of serotype 2a strains to Sf6, and showed that serotype 2a strains have better invasion ability. Lastly, we revealed two new serotype conversions for S. flexneri , thereby contributing to understanding the evolution of this important human pathogen. IMPORTANCE The emergence of antibiotic-resistant strains and lack of efficient vaccines have made Shigella a priority organism for the World Health Organization (1). Therefore, bacteriophage therapy has received increasing attention as an alternative therapeutic approach. LPS Oag is the most variable part of LPS due to chemical modifications and is the target of bacteriophage Sf6 ( S. flexneri specific). We dissected the evolution of S. flexneri serotype Y to 2a 2 , which revealed a new role for a gene acquired during serotype conversion and furthermore identified new specific forms of LPS receptor for Sf6. Collectively, these results unfold the importance of the acquisition of those Oag modification genes and further our understanding of the relationship between Sf6 and S. flexneri .
(Copyright © 2020 American Society for Microbiology.) |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
