A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion.

Autor: Kratasyuk VA; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia.; Federal Research Center 'Krasnoyarsk Science Center SB RAS', Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russia., Stepanova LV; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia., Ranjan R; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia., Sutormin OS; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia., Pande S; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia.; Krasnoyarsk State Medical University named after professor V.F.Voyno-Yasenetsky of the Ministry of Health of the Russian Federation, Av. Partizan Zheleznyak 1, Krasnoyarsk, Russia., Zhukova GV; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia., Miller OM; Krasnoyarsk Maternity and Childhood Protection Center, Kirenskogo Street 2a, Krasnoyarsk, Russia., Maznyak NV; School of Non-Ferrous Metals and Materials Science, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia., Kolenchukova OA; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny prospect 79, Krasnoyarsk, Russia.; Scientific Research Institute of Medical Problems of the North, Av. Partizan Zheleznyak 3g, Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2021 Mar; Vol. 36 (2), pp. 384-390. Date of Electronic Publication: 2020 Nov 03.
DOI: 10.1002/bio.3954
Abstrakt: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I exp , mean maximum bioluminescence intensity of the experimental measurement (I exp ); I c , luminescence intensity in control; I exp /I c , %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.
(© 2020 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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