NOD2 receptor is crucial for protecting against the digestive form of Chagas disease.

Autor: Pereira NS; Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, Brazil.; Potiguar University, Natal, Brazil., Queiroga TBD; Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Brazil., da Silva DD; Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Brazil., Nascimento MSL; Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Brazil., Andrade CM; Department of Biomedical Sciences, University of Rio Grande do Norte State, Mossoró, Brazil., Souto JT; Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Brazil., Ricci MF; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Arantes RME; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Zamboni DS; Department of Cellular and Molecular Biology and Pathogenic Bioagents, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Chiari E; Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Câmara ACJD; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte, Natal, Brazil., Galvão LMDC; Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, Brazil.; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte, Natal, Brazil., Guedes PMM; Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, Brazil.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2020 Sep 28; Vol. 14 (9), pp. e0008667. Date of Electronic Publication: 2020 Sep 28 (Print Publication: 2020).
DOI: 10.1371/journal.pntd.0008667
Abstrakt: Digestive and cardiodigestive forms of Chagas' disease are observed in 2% to 27% of the patients, depending on their geographic location, Trypanosoma cruzi strain and immunopathological responses. The aim of this work was to evaluate the role of NOD2 innate immune receptor in the pathogenesis of the digestive system in Chagas' disease. Patients with digestive form of the disease showed lower mRNA expression of NOD2, higher expression of RIP2 and α-defensin 6, compared to indeterminate form, detected by Real-time PCR in peripheral blood mononuclear cells. In addition, there was a negative correlation between the expression of NOD2 and the degree of dilation of the esophagus, sigmoid and rectum in those patients. The infection of NOD2-/- mice with T. cruzi strain isolated from the digestive patient induced a decrease in intestinal motility. Histopathological analysis of the colon and jejunum of NOD2-/- and wild type C57BL/6 animals revealed discrete inflammatory foci during the acute phase of infection. Interestingly, during the chronic phase of the infection there was inflammation and hypertrophy of the longitudinal and circular muscular layer more pronounced in the colon and jejunum from NOD2-/- animals, when compared to wild type C57BL/6 mice. Together, our results suggest that NOD2 plays a protective role against the development of digestive form of Chagas' disease.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje