A Role for Astroglial Calcium in Mammalian Sleep and Sleep Regulation.

Autor:	Ingiosi AM; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA., Hayworth CR; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA., Harvey DO; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA., Singletary KG; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA., Rempe MJ; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA; Department of Mathematics and Computer Science, Whitworth University, West Hawthorne Road, Spokane, WA 99251, USA., Wisor JP; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA., Frank MG; Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, East Spokane Falls Boulevard, Spokane, WA 99202, USA. Electronic address: marcos.frank@wsu.edu.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2020 Nov 16; Vol. 30 (22), pp. 4373-4383.e7. Date of Electronic Publication: 2020 Sep 24.
DOI:	10.1016/j.cub.2020.08.052
Abstrakt:	Mammalian sleep expression and regulation have historically been thought to reflect the activity of neurons. Changes in other brain cells (glia) across the sleep-wake cycle and their role in sleep regulation are comparatively unexplored. We show that sleep and wakefulness are accompanied by state-dependent changes in astroglial activity. Using a miniature microscope in freely behaving mice and a two-photon microscope in head-fixed, unanesthetized mice, we show that astroglial calcium signals are highest in wake and lowest in sleep and are most pronounced in astroglial processes. We also find that astroglial calcium signals during non-rapid eye movement sleep change in proportion to sleep need. In contrast to neurons, astrocytes become less synchronized during non-rapid eye movement sleep after sleep deprivation at the network and single-cell level. Finally, we show that conditionally reducing intracellular calcium in astrocytes impairs the homeostatic response to sleep deprivation. Thus, astroglial calcium activity changes dynamically across vigilance states, is proportional to sleep need, and is a component of the sleep homeostat. Competing Interests: Declaration of Interests The authors declare no competing interests. (Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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