| Autor: |
Westerbeek EY; μFlow Group, Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium. Wim.De.Malsche@vub.be., Bomer JG, Olthuis W, Eijkel JCT, De Malsche W |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2020 Nov 07; Vol. 20 (21), pp. 3938-3947. Date of Electronic Publication: 2020 Sep 25. |
| DOI: |
10.1039/d0lc00773k |
| Abstrakt: |
Chromatographic columns are suffering from Taylor-Aris dispersion, especially for slowly diffusing molecules such as proteins. Since downscaling the channel size to reduce Taylor-Aris dispersion meets fundamental pressure limitations, new strategies are needed to further improve chromatography beyond its current limits. In this work we demonstrate a method to reduce Taylor-Aris dispersion by lateral mixing in a newly designed silicon AC-electroosmotic flow mixer. We obtained a reduction in κ aris by a factor of three in a 40 μm × 20 μm microchannel, corresponding to a plate height gain of 2 to 3 under unretained conditions at low to high Pe values. We also demonstrate an improvement of a reverse-phase chromatographic separation of coumarins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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