Shiga toxin-producing Escherichia coli O26:H11 associated with a cluster of haemolytic uraemic syndrome cases in South Africa, 2017.

Autor:	Smith AM; Centre for Enteric Diseases, National Institute for Communicable Diseases (NICD), National Health Laboratory Service (NHLS), Johannesburg, South Africa.; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Tau NP; Centre for Enteric Diseases, National Institute for Communicable Diseases (NICD), National Health Laboratory Service (NHLS), Johannesburg, South Africa., Kalule BJ; Division of Medical Microbiology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa., Nicol MP; Division of Medical Microbiology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.; School of Biomedical Sciences, University of Western Australia, Perth, Australia., McCulloch M; Red Cross Children's Hospital, University of Cape Town, Cape Town, South Africa., Jacobs CA; Communicable Disease Control, Department of Health, Cape Town, South Africa., McCarthy KM; Division of Public Health Surveillance and Response, NICD, NHLS, Johannesburg, South Africa., Ismail A; Sequencing Core Facility, NICD, NHLS, Johannesburg, South Africa., Allam M; Sequencing Core Facility, NICD, NHLS, Johannesburg, South Africa., Kleynhans J; South African Field Epidemiology Training Programme, NICD, NHLS, Johannesburg, South Africa.
Jazyk:	angličtina
Zdroj:	Access microbiology [Access Microbiol] 2019 Sep 12; Vol. 1 (9), pp. e000061. Date of Electronic Publication: 2019 Sep 12 (Print Publication: 2019).
DOI:	10.1099/acmi.0.000061
Abstrakt:	Introduction: Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that may cause diarrhoeal outbreaks and occasionally are associated with haemolytic-uraemic syndrome (HUS). We report on STEC O26:H11 associated with a cluster of four HUS cases in South Africa in 2017. Methodology: All case-patients were female and aged 5 years and under. Standard microbiological tests were performed for culture and identification of STEC from specimens (human stool and food samples). Further analysis of genomic DNA extracted from bacterial cultures and specimens included PCR for specific virulence genes, whole-genome sequencing and shotgun metagenomic sequencing. Results: For 2/4 cases, stool specimens revealed STEC O26:H11 containing eae , stx2a and stx2b virulence genes. All food samples were found to be negative for STEC. No epidemiological links could be established between the HUS cases. Dried meat products were the leading food item suspected to be the vehicle of transmission for these cases, as 3/4 case-patients reported they had eaten this. However, testing of dried meat products could not confirm this. Conclusion: Since STEC infection does not always lead to severe symptoms, it is possible that many more cases were associated with this cluster and largely went unrecognized. Competing Interests: The authors declare that there are no conflicts of interest. (© 2019 The Authors.)
Databáze:	MEDLINE
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