Validating an Automated Nucleic Acid Extraction Device for Omics in Space Using Whole Cell Microbial Reference Standards.

Autor:	Urbaniak C; NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Wong S; AI Biosciences, College Station, TX, United States., Tighe S; University of Vermont, Burlington, VT, United States., Arumugam A; AI Biosciences, College Station, TX, United States., Liu B; AI Biosciences, College Station, TX, United States., Parker CW; NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Wood JM; NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Singh NK; NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Skorupa DJ; Montana State University, Bozeman, MT, United States., Peyton BM; Montana State University, Bozeman, MT, United States., Jenson R; IRPI LCC, Portland, OR, United States., Karouia F; NASA Ames Research Center, Moffett Field, CA, United States., Dragon J; University of Vermont, Burlington, VT, United States., Venkateswaran K; NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Aug 21; Vol. 11, pp. 1909. Date of Electronic Publication: 2020 Aug 21 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.01909
Abstrakt:	NASA has made great strides in the past five years to develop a suite of instruments for the International Space Station in order to perform molecular biology in space. However, a key piece of equipment that has been lacking is an instrument that can extract nucleic acids from an array of complex human and environmental samples. The Omics in Space team has developed the μTitan (simulated micro( μ ) gravity t ested i ns t rument for a utomated n ucleic acid) system capable of automated, streamlined, nucleic acid extraction that is adapted for use under microgravity. The μTitan system was validated using a whole cell microbial reference (WCMR) standard comprised of a suspension of nine bacterial strains, titrated to concentrations that would challenge the performance of the instrument, as well as to determine the detection limits for isolating DNA. Quantitative assessment of system performance was measured by comparing instrument input challenge dose vs recovery by Qubit spectrofluorometry, qPCR, Bioanalyzer, and Next Generation Sequencing. Overall, results indicate that the μTitan system performs equal to or greater than a similar commercially available, earth-based, automated nucleic acid extraction device. The μTitan system was also tested in Yellowstone National Park (YNP) with the WCMR, to mimic a remote setting, with limited resources. The performance of the device at YNP was comparable to that in a laboratory setting. Such a portable, field-deployable, nucleic extraction system will be valuable for environmental microbiology, as well as in health care diagnostics. (Copyright © 2020 Urbaniak, Wong, Tighe, Arumugam, Liu, Parker, Wood, Singh, Skorupa, Peyton, Jenson, Karouia, Dragon and Venkateswaran.)
Databáze:	MEDLINE
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