Sugarcane bagasse hydrolysates as feedstock to produce the isopropanol-butanol-ethanol fuel mixture: Effect of lactic acid derived from microbial contamination on Clostridium beijerinckii DSM 6423.

Autor: Vieira CFDS; Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Codogno MC; Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Maugeri Filho F; Bioprocess and Metabolic Engineering Laboratory (LEMeB), School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Maciel Filho R; Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Mariano AP; Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: adpm@unicamp.br.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2021 Jan; Vol. 319, pp. 124140. Date of Electronic Publication: 2020 Sep 17.
DOI: 10.1016/j.biortech.2020.124140
Abstrakt: Enzymatic hydrolysis of lignocellulose under industrial conditions is prone to contamination by lactic acid bacteria, and in this study, a cellulose hydrolysate produced from dilute-acid pretreatedsugarcane bagasse contained 13 g/L lactic acid and was used for IBE production by Clostridium beijerinckii DSM 6423. In fermentation of the cellulose hydrolysate supplemented with sugarcane molasses for nutrients and buffering of the medium (40 g/L total sugar), 92% of the lactic acid was consumed, and the butanol yield was as high as 0.28 (7.9 g/L butanol), suggesting that lactic acid was preferentially metabolized to butanol. When the hydrolysate was mixed with a detoxified bagasse hemicellulose hydrolysate and supplemented with molasses (35 g/L total sugar), the culture was able to exhaust glucose and utilized sucrose (by 38%), xylose (31%), and lactic acid (70%). Overall, this study shows that C. beijerinckii DSM 6423 can co-ferment first- and second-generation sugars while consuming lactic acid.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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