Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish.

Autor: Charlie-Silva I; Department of Pharmacology at University of São Paulo-ICB/USP, São Paulo-SP, Brazil., Feitosa NM; Laboratório Integrado de Biociências Translacionais (LIBT), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ)- Macaé, RJ, Brazil., Gomes JMM; Department of Morphology, ICB-UFMG, Belo Horizonte-MG, Brazil., Hoyos DCM; Aquiculture Laboratory (Laqua), UFMG, Belo Horizonte-MG, Brazil., Mattioli CC; Department of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal-SP, Brazil., Eto SF; Department of Postgraduate in Health Sciences-PROCISA, Federal University of Roraima (UFRR), Boa Vista, Brazil., Fernandes DC; Institute of Chemistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil., Belo MAA; Department of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal-SP, Brazil.; Laboratory of Animal Pharmacology and Toxicology, Brasil University, Descalvado/SP, Brazil., Silva JO; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy-UFMG, Belo Horizonte-MG, Brazil., Barros ALB; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy-UFMG, Belo Horizonte-MG, Brazil., Corrêa Junior JD; Department of Morphology, ICB-UFMG, Belo Horizonte-MG, Brazil., de Menezes GB; Department of Morphology, ICB-UFMG, Belo Horizonte-MG, Brazil., Fukushima HCS; Laboratory of Applied Immunology, Federal University of São Carlos, São Carlos-SP, Brazil., Castro TFD; Laboratory of Applied Immunology, Federal University of São Carlos, São Carlos-SP, Brazil., Borra RC; Laboratory of Applied Immunology, Federal University of São Carlos, São Carlos-SP, Brazil., Pierezan F; School of Veterinary Medicine, Department of Clinic and Veterinary Surgery, UFMG, Belo Horizonte-MG, Brazil., de Melo NFS; Faculty of Medicine São Leopoldo Mandic, Araras-SP, Brazil., Fraceto LF; Institute of Science and Technology of Sorocaba, São Paulo State University, Sorocaba-SP, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Sep 24; Vol. 15 (9), pp. e0238823. Date of Electronic Publication: 2020 Sep 24 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0238823
Abstrakt: Mucoadhesive polymeric nanocapsules have attracted interest of researchers from different fields from natural sciences because of their ability to interact with the mucosa and increase drug permeation. Anesthesia by immersion causes absorption through the skin and gills of fish, so it is important to evaluate the exposure of these organs to drug nanosystems. Benzocaine (BENZ) is one of the most popular anesthetic agents used in fish anesthesia, but it has drawbacks because of its low bioavailability, resulting in weak absorption after immersion. Here we describe method developed for preparing and characterizing chitosan-coated PLGA mucoadhesive nanoparticles containing BENZ (NPMAs) for zebrafish immersion anesthesia. We determined the lowest effective concentration, characterized the interaction of the mucoadhesive system with fish, measured the anesthetic efficacy, and evaluated possible toxic effects in embryos and adults exposed to the nanoformulations. This study opens perspectives for using nanoformulations prepared with BENZ in aquaculture, allowing reduction of dosage as well as promoting more effective anesthesia and improved interaction with the mucoadhesive system of fish.
Competing Interests: The authors have read the journal’s policy and have the following potential competing interests: authors LFF and GBdM are members of the PLOS ONE Editorial Board. This does not alter our adherence to PLOS ONE policies on sharing data and materials. There are no patents, products in development or marketed products associated with this research to declare.
Databáze: MEDLINE
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