Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase.

Autor: Bogoutdinova LR; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Lazareva EM; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.; Biology Faculty M.V. Lomonosov Moscow State University, Leninskie Gory 1, building 40, 119991 Moscow, Russia., Chaban IA; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Kononenko NV; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Dilovarova T; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Khaliluev MR; Plant Cell Engineering Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.; Agronomy and Biotechnology Faculty of Moscow Timiryazev Agricultural Academy, Russian State Agrarian University, Timiryazevskaya 49, 127550 Moscow, Russia., Kurenina LV; Plant Cell Engineering Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Gulevich AA; Plant Cell Engineering Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Smirnova EA; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.; Biology Faculty M.V. Lomonosov Moscow State University, Leninskie Gory 1, building 40, 119991 Moscow, Russia., Baranova EN; Plant Cell Biology Laboratory All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.; N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, 127276 Moscow, Russia.
Jazyk: angličtina
Zdroj: Biology [Biology (Basel)] 2020 Sep 18; Vol. 9 (9). Date of Electronic Publication: 2020 Sep 18.
DOI: 10.3390/biology9090297
Abstrakt: Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage the cellular structures. The engineering of transgenic plants with higher production of ROS-scavenging enzyme in plant cells could protect the integrity of such a fine intracellular structure as the cytoskeleton and each cellular compartment. We analyzed the morphological changes in root tip cells caused by the application of iso-osmotic NaCl and Na 2 SO 4 solutions to tomato plants harboring an introduced superoxide dismutase gene. To study the roots of tomato plants cultivar Belyi Naliv (WT) and FeSOD-transgenic line, we examined the distribution of ROS and enzyme-linked immunosorbent detection of α-tubulin. In addition, longitudinal sections of the root apexes were compared. Transmission electronic microscopy of atypical cytoskeleton structures was also performed. The differences in the microtubules cortical network between WT and transgenic plants without salt stress were detected. The differences were found in the cortical network of microtubules between WT and transgenic plants in the absence of salt stress. While an ordered microtubule network was revealed in the root cells of WT tomato, no such degree of ordering was detected in transgenic line cells. The signs of microtubule disorganization in root cells of WT plants were manifested under the NaCl treatment. On the contrary, the cytoskeleton structural organization in the transgenic line cells was more ordered. Similar changes, including the cortical microtubules disorganization, possibly associated with the formation of atypical tubulin polymers as a response to salt stress caused by Na 2 SO 4 treatment, were also observed. Changes in cell size, due to both vacuolization and impaired cell expansion in columella zone and cap initials, were responsible for the root tip tissue modification.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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