Proliferative and chondrogenic potential of mesenchymal stromal cells from pluripotent and bone marrow cells.

Autor:	Sfougataki I; Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece.; Research Institute for the Study of Genetic and Malignant Disorders in Childhood, Aghia Sophia Children's Hospital, Athens, Greece. irene_sf_@hotmail.com., Varela I; Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece., Stefanaki K; Department of Histopathology, Aghia Sophia Children's Hospital, Athens, Greece., Karagiannidou A; Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece., Roubelakis MG; Laboratory of Biology, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Kalodimou V; Flow Cytometry-Research and Regenerative Medicine Department, IASO Hospital, Athens, Greece., Papathanasiou I; Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, University of Thessally, Thessally, Greece., Traeger-Synodinos J; Department of Medical Genetics, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Kitsiou-Tzeli S; Department of Medical Genetics, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Kanavakis E; Genesis Genoma Lab, Genetic diagnosis, Clinical Genetics and Research, Chalandri, Greece., Kitra V; Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece., Tsezou A; Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, University of Thessally, Thessally, Greece., Tzetis M; Department of Medical Genetics, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Goussetis E; Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece.
Jazyk:	angličtina
Zdroj:	Histology and histopathology [Histol Histopathol] 2020 Dec; Vol. 35 (12), pp. 1415-1426. Date of Electronic Publication: 2020 Sep 22.
DOI:	10.14670/HH-18-259
Abstrakt:	Introduction: Mesenchymal stromal cells (MSCs) can be derived from a wide range of fetal and adult sources including pluripotent stem cells (PSCs). The properties of PSC-derived MSCs need to be fully characterized, in order to evaluate the feasibility of their use in clinical applications. PSC-MSC proliferation and differentiation potential in comparison with bone marrow (BM)-MSCs is still under investigation. The objective of this study was to determine the proliferative and chondrogenic capabilities of both human induced pluripotent stem cell (hiPSC-) and embryonic stem cell (hESC-) derived MSCs, by comparing them with BM-MSCs. Methods: MSCs were derived from two hiPSC lines (hiPSC-MSCs), the well characterized Hues9 hESC line (hESC-MSCs) and BM from two healthy donors (BM-MSCs). Proliferation potential was investigated using appropriate culture conditions, with serial passaging, until cells entered into senescence. Differentiation potential to cartilage was examined after in vitro chondrogenic culture conditions. Results: BM-MSCs revealed a fold expansion of 1.18x10⁵ and 2.3x10⁵ while the two hiPSC-MSC lines and hESC-MSC showed 5.88x10¹⁰, 3.49x10⁸ and 2.88x10⁸, respectively. Under chondrogenic conditions, all MSC lines showed a degree of chondrogenesis. However, when we examined the formed chondrocyte micromasses by histological analysis of the cartilage morphology and immunohistochemistry for the chondrocyte specific markers Sox9 and Collagen II, we observed that PSC-derived MSC lines had formed pink rather than hyaline cartilage, in contrast to BM-MSCs. Conclusion: In conclusion, MSCs derived from both hESCs and hiPSCs had superior proliferative capacity compared to BM-MSCs, but they were inefficient in their ability to form hyaline cartilage.
Databáze:	MEDLINE
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