Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na + , K + )-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae).

Autor: Leone FA; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: fdaleone@ffclrp.usp.br., Lucena MN; Instituto de Biociências, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil., Fabri LM; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil., Garçon DP; Universidade Federal do Triângulo Mineiro, Iturama, MG, Brazil., Fontes CFL; Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Brazil., Faleiros RO; Departamento de Ciências Agrárias e Biológicas, Universidade Federal do Espírito Santo, São Mateus, ES, Brazil., Moraes CM; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil., McNamara JC; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Centro de Biologia Marinha, Universidade de São Paulo, São Sebastião, SP, Brazil.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2020 Dec; Vol. 250, pp. 110507. Date of Electronic Publication: 2020 Sep 18.
DOI: 10.1016/j.cbpb.2020.110507
Abstrakt: We analyzed the modulation by exogenous FXYD2 peptide and by endogenous protein kinases A and C, and Ca 2+ -calmodulin-dependent kinase, of gill (Na + , K + )-ATPase activity in the semi-terrestrial mangrove crab Ucides cordatus after 10-days acclimation to different salinities. Osmotic and ionic regulatory ability and gill (Na + , K + )-ATPase activity also were evaluated. (Na + , K + )-ATPase activity is stimulated by exogenous pig kidney FXYD2 peptide, while phosphorylation by endogenous protein kinases A and C and Ca 2+ /calmodulin-dependent kinase inhibits activity. Stimulation by FXYD2 and inhibition by protein kinase C and Ca 2+ /calmodulin-dependent kinase are salinity-dependent. This is the first demonstration of inhibitory phosphorylation of a crustacean (Na + , K + )-ATPase by Ca 2+ /calmodulin-dependent kinase. At low salinities, the (Na + , K + )-ATPase exhibited a single, low affinity ATP-binding site that showed Michaelis-Menten behavior. Above 18‰S, a second, cooperative, high affinity ATP-binding site appeared, corresponding to 10-20% of total (Na + , K + )-ATPase activity. Hemolymph osmolality was strongly hyper-/hypo-regulated in crabs acclimated at 2 to 35‰S. Cl - was well hyper-/hypo-regulated although Na + much less so, becoming isonatremic at elevated salinity. (Na + , K + )-ATPase activity was greatest in isosmotic crabs (26‰S), decreasing notably at 35‰S and also diminishing progressively from 18to 2‰S. Hyper-osmoregulation in U. cordatus showed little dependence on gill (Na + , K + )-ATPase activity, suggesting a role for other ion transporters. These findings reveal that the salinity acclimation response in U. cordatus consists of a suite of enzymatic and osmoregulatory adjustments that maintain its osmotic homeostasis in a challenging, mangrove forest environment.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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