Antimicrobial and antibiofilm activity of the benzoquinone oncocalyxone A.

Autor: da Silva RE; Center of Research in Medicinal Plants - NPPM, Federal University of Piauí - UFPI, Teresina, Piauí, Brazil., Ribeiro FOS; Biotechnology and Biodiversity Center Research - BIOTEC, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil; Center for Research in Applied Morphology and Immunology, NuPMIA, University of Brasilia, Brasilia, Brazil., de Carvalho AMA; Group of Advanced Studies in Medical Mycology - GEAMICOL, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil., Daboit TC; Group of Advanced Studies in Medical Mycology - GEAMICOL, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil., Marinho-Filho JDB; Delta Cell Culture Laboratory - LCCDelta, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil., Matos TS; Department of Organic and Inorganic Chemistry, Federal University of Ceará - UFC, Fortaleza, Ceará, Brazil., Pessoa ODL; Department of Organic and Inorganic Chemistry, Federal University of Ceará - UFC, Fortaleza, Ceará, Brazil., de Souza de Almeida Leite JR; Biotechnology and Biodiversity Center Research - BIOTEC, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil; Center for Research in Applied Morphology and Immunology, NuPMIA, University of Brasilia, Brasilia, Brazil., de Araújo AR; Biotechnology and Biodiversity Center Research - BIOTEC, Federal University of Delta do Parnaíba - UFDPar, Parnaíba, Piauí, Brazil. Electronic address: alyne.rdearaujo@gmail.com., Dos Santos Soares MJ; Center of Research in Medicinal Plants - NPPM, Federal University of Piauí - UFPI, Teresina, Piauí, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2020 Dec; Vol. 149, pp. 104513. Date of Electronic Publication: 2020 Sep 17.
DOI: 10.1016/j.micpath.2020.104513
Abstrakt: Resistance to antimicrobials is a challenging issue that complicates the treatment of infections caused by bacteria and fungi, thus requiring new therapeutic options. Oncocalyxone A, a benzoquinone obtained from Auxemma oncocalyx (Allem) Taub has several biological effects; however, there is no data on its antimicrobial action. In this study, its antimicrobial and antibiofilm activities were evaluated against bacteria and fungi of clinical interest. Strains of Gram-positive and Gram-negative bacteria, and filamentous fungi and yeasts were selected to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of oncocalyxone A. The antibacterial effect of oncocalyxone A was studied using survival curves, atomic force microscopy (AFM), and the involvement of oxidative stress. We examined the inhibitory action of the molecule on biofilm formation and its hemolytic activity against human erythrocytes. Our results showed that among the strains tested, Staphylococcus epidermidis was highly sensitive to the action of oncocalyxone A, with an MIC of 9.43 μg/mL. In most bacterial strains analyzed, a bacteriostatic effect was observed, though the molecule showed no antifungal activity. Antibiofilm activity was observed against the methicillin-resistant S. aureus bacteria. Additionally, results from atomic force microscopy imaging showed that oncocalyxone A significantly altered bacterial morphology. Further, oncocalyxone A showed no hemolytic activity at concentrations ≥151 μg/mL. Together, our results demonstrate the antibacterial and antibiofilm potential of oncocalyxone A, indicating its therapeutic potential against bacterial resistance.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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