Extraction, characterization of xylan from Azadirachta indica (neem) sawdust and production of antiproliferative xylooligosaccharides.

Autor: Sharma K; Carbohydrate Enzyme Biotechnology Laboratory, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Morla S; Viral Immunology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Khaire KC; Center for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Thakur A; Carbohydrate Enzyme Biotechnology Laboratory, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Moholkar VS; Center for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Kumar S; Viral Immunology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India., Goyal A; Carbohydrate Enzyme Biotechnology Laboratory, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India; Center for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India. Electronic address: arungoyl@iitg.ac.in.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Nov 15; Vol. 163, pp. 1897-1907. Date of Electronic Publication: 2020 Sep 15.
DOI: 10.1016/j.ijbiomac.2020.09.086
Abstrakt: Xylan extracted from neem sawdust gave 22.5%, (w/w) yield. The extracted xylan was composed of xylose and glucuronic acid at a molar ratio of 8:1 and with a molecular mass, ~66 kDa. FTIR and NMR analyses indicated a backbone of xylan substituted with 4-O-methyl glucuronic acid at the O-2 position. FESEM analysis showed a highly porous and granular surface structure of xylan. A thermogravimetric study of xylan showed thermal denaturation at 271 °C. The hydrolysis of xylan by recombinant endo-β-1,4-xylanase produced a mixture of xylooligosaccharides ranging from degree of polymerization 2-7. Xylooligosaccharides inhibited cell growth of human colorectal cancer (HT-29) cells but did not affect the mouse fibroblast cells confirming its biocompatibility. Western blotting, DNA laddering and flow cytometric analysis displayed inhibition of HT-29 cells by xylooligosaccharides. FLICA staining and mitochondrial membrane potential analyses confirmed the activation of the intrinsic pathway of apoptosis. The study amply indicated that the xylooligosaccharides produced from neem xylan could be potentially used as an antiproliferative agent.
Competing Interests: Declaration of competing interest The authors do not have a potential conflict of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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