Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms.

Autor: Li X; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Chan JF; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.; Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China., Li KK; Department of Ophthalmology, United Christian Hospital, Hong Kong Special Administrative Region, China., Tso EY; Department of Medicine, United Christian Hospital, Hong Kong Special Administrative Region, China., Yip CC; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Sridhar S; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China., Chung TW; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Chiu KH; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Hung DL; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Wu AK; Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Hong Kong Special Administrative Region, China., Chau SK; Department of Pathology, United Christian Hospital, Hong Kong Special Administrative Region, China., Liu R; Department of Medicine, Ruttonjee and Tang Shiu Kin Hospital, Hong Kong Special Administrative Region, China., Lung KC; Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong Special Administrative Region, China., Tam AR; Department of Medicine, Queen Mary Hospital, Hong Kong Special Administrative Region, China., Cheng VC; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China., To KK; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China., Chan KH; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China., Hung IF; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China. ivanhung@hku.hk.; Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China. ivanhung@hku.hk.; Department of Medicine, Queen Mary Hospital, Hong Kong Special Administrative Region, China. ivanhung@hku.hk., Yuen KY; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.; Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.
Jazyk: angličtina
Zdroj: Infection [Infection] 2021 Apr; Vol. 49 (2), pp. 257-265. Date of Electronic Publication: 2020 Sep 17.
DOI: 10.1007/s15010-020-01524-2
Abstrakt: Purpose: To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
Methods: Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
Results: A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2-27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
Conclusion: Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.
Databáze: MEDLINE
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