Design of Chitosan Nanocapsules with Compritol 888 ATO® for Imiquimod Transdermal Administration. Evaluation of Their Skin Absorption by Raman Microscopy.

Autor: Alvarez-Figueroa MJ; Facultad de Química y de Farmacia, Departamento de Farmacia, Pontificia Universidad Católica de Chile, Vicuña Mackena 4860, CP: 7820436, Macul, Santiago, Chile. mjalvare@uc.cl., Narváez-Araya D; Facultad de Química y de Farmacia, Departamento de Farmacia, Pontificia Universidad Católica de Chile, Vicuña Mackena 4860, CP: 7820436, Macul, Santiago, Chile., Armijo-Escalona N; Facultad de Química y de Farmacia, Departamento de Farmacia, Pontificia Universidad Católica de Chile, Vicuña Mackena 4860, CP: 7820436, Macul, Santiago, Chile., Carrasco-Flores EA; Laboratorio de Espectroscopia Vibracional, Facultad de Ciencias, Universidad de Chile, Santiago, Chile., González-Aramundiz JV; Facultad de Química y de Farmacia, Departamento de Farmacia, Pontificia Universidad Católica de Chile, Vicuña Mackena 4860, CP: 7820436, Macul, Santiago, Chile. jvgonzal@uc.cl.; Centro de Investigación en Nanotecnología y Materiales Avanzados 'CIEN-UC', Pontificia Universidad Católica de Chile, Santiago, Chile. jvgonzal@uc.cl.
Jazyk: angličtina
Zdroj: Pharmaceutical research [Pharm Res] 2020 Sep 17; Vol. 37 (10), pp. 195. Date of Electronic Publication: 2020 Sep 17.
DOI: 10.1007/s11095-020-02925-6
Abstrakt: Purpose: Design imiquimod-loaded chitosan nanocapsules for transdermal delivery and evaluate the depth of imiquimod transdermal absorption as well as the kinetics of this absorption using Raman Microscopy, an innovative strategy to evaluate transdermal absorption. This nanovehicle included Compritol 888ATO®, a novel excipient for formulating nanosystems whose administration through the skin has not been studied until now.
Methods: Nanocapsules were made by solvent displacement method and their physicochemical properties was measured by DLS and laser-Doppler. For transdermal experiments, newborn pig skin was used. The Raman spectra were obtained using a laser excitation source at 532 nm and a 20/50X oil immersion objective.
Results: The designed nanocapsules, presented nanometric size (180 nm), a polydispersity index <0.2 and a zeta potential +17. The controlled release effect of Compritol was observed, with the finding that half of the drug was released at 24 h in comparison with control (p < 0.05). It was verified through Raman microscopy that imiquimod transdermal penetration is dynamic, the nanocapsules take around 50 min to penetrate the stratum corneum and 24 h after transdermal administration, the drug was in the inner layers of the skin.
Conclusions: This study demonstrated the utility of Raman Microscopy to evaluate the drugs transdermal penetration of in the different layers of the skin. Graphical Abstract New imiquimod nanocapsules: evaluation of their skin absorption by Raman Microscopy and effect of the compritol 888ATO® in the imiquimod release profile.
Databáze: MEDLINE
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