CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay.

Autor:	Conti S; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute for Science and Technology (BIST), Barcelona, Spain., Kato T; The Francis Crick Institute, London, UK., Park D; The Francis Crick Institute, London, UK., Sahai E; The Francis Crick Institute, London, UK., Trepat X; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute for Science and Technology (BIST), Barcelona, Spain. xtrepat@ibecbarcelona.eu.; Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain. xtrepat@ibecbarcelona.eu.; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. xtrepat@ibecbarcelona.eu.; Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. xtrepat@ibecbarcelona.eu., Labernadie A; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute for Science and Technology (BIST), Barcelona, Spain. alabernadie@ibecbarcelona.eu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2179, pp. 243-256.
DOI:	10.1007/978-1-0716-0779-4_19
Abstrakt:	In many solid tumors, collective cell invasion prevails over single-cell dissemination strategies. Collective modes of invasion often display specific front/rear cellular organization, where invasive leader cells arise from cancer cell populations or the tumor stroma. Collective invasion involves coordinated cellular movements which require tight mechanical crosstalk through specific combinations of cell-cell interactions and cell-matrix adhesions. Cancer Associated Fibroblasts (CAFs) have been recently reported to drive the dissemination of epithelial cancer cells through ECM remodeling and direct intercellular contact. However, the cooperation between tumor and stromal cells remains poorly understood. Here we present a simple spheroid invasion assay to assess the role of CAFs in the collective migration of epithelial tumor cells. This method enables the characterization of 3D spheroid invasion patterns through live cell fluorescent labeling combined with spinning disc microscopy. When embedded in extracellular matrix, the invasive strands of spheroids can be tracked and leader/follower organization of CAFs and cancer cells can be quantified.
Databáze:	MEDLINE
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