| Autor: |
Bhattacharya D; School of Environmental Studies, Jadavpur University, Kolkata, 700 032, India., de Los Santos Villalobos S; Instituto Tecnológico de Sonora, 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Ciudad Obregón, Sonora, Mexico., Ruiz VV; Instituto Tecnológico de Sonora, 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Ciudad Obregón, Sonora, Mexico., Selvin J; Department of Microbiology, Pondicherry University, Puducherry, 605 014, India., Mukherjee J; School of Environmental Studies, Jadavpur University, Kolkata, 700 032, India. joydeep.mukherjee@jadavpuruniversity.in. |
| Jazyk: |
angličtina |
| Zdroj: |
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2020 Nov; Vol. 113 (11), pp. 1675-1687. Date of Electronic Publication: 2020 Sep 16. |
| DOI: |
10.1007/s10482-020-01472-9 |
| Abstrakt: |
A novel Gram-positive and endospore-forming bacterium assigned as strain SPB7 T which is also a new source of a cyclic diketopiperazine (3S,6S)-3,6-diisobutylpiperazine-2,5-dione is described. A polyphasic (biochemical, phenotypic and genotypic) approach was used to clarify the taxonomic affiliation of this strain. The partial and complete 16S rRNA gene sequences revealed that strain SPB7 T is a member of the Bacillus genus [showing high similarity (> 98.70%) with Bacillus spizizenii NRRL B-23049 T , Bacillus tequilensis KCTC 13622 T , Bacillus inaquosorum KCTC 13429 T and Bacillus cabrialesii TE3 T ]. The maximum values for average nucleotide identity (ANI) and in silico DNA-DNA hybridization (GGDC, Formula 2) of strain SPB7 T was obtained for twenty-five strains of Bacillus spizizenii (ANI 95.01-95.48% and GGDC 62.70-60.00%). The whole-genome phylogenetic relationship showed that SPB7 T formed an individual and separated clade with the Bacillus spizizenii group. Principal cellular fatty acids identified in strain SPB7 T were anteiso C 15:0 , anteiso C 17:0 , iso C 15:0 , iso C 17:0 , C 16:0 , C 10:0 3OH and iso C 17:1 ϖ10c . Polar lipid profile showed presence of diphosphotidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and five unknown lipids. Cells were rod shaped, catalase, oxidase-positive and motile. Growth occurred at 20-45 °C (optimal 35 °C), at pH 6.0-10.0 (optimal pH 8) and 0-10% (w/v) NaCl (optimal 2%). The phenotypic, biochemical, and genotypic traits of strain SPB7 T strongly supported its taxonomic affiliation as a novel species of the Bacillus genus, for which the name Bacillus rugosus sp. nov. is proposed. The type strain is SPB7 T (= NRRL B-65559 T , = CICC 24827 T , = MCC 4185 T ). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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