Aptamer-based electrochemical biosensing strategy toward human non-small cell lung cancer using polyacrylonitrile/polypyrrole nanofibers.

Autor: Kivrak E; Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100, Izmir, Bornova, Turkey.; Graduate School of Natural and Applied Sciences, Department of Biomedical Technologies, Ege University, 35100, Izmir, Bornova, Turkey., Ince-Yardimci A; Izmir Institute of Technology, Department of Chemical Engineering, 35430 Urla, İzmir, Turkey., Ilhan R; Faculty of Pharmacy, Department of Biochemistry, Ege University, 35100, Izmir, Bornova, Turkey., Kirmizibayrak PB; Faculty of Pharmacy, Department of Biochemistry, Ege University, 35100, Izmir, Bornova, Turkey., Yilmaz S; Izmir Institute of Technology, Department of Chemical Engineering, 35430 Urla, İzmir, Turkey., Kara P; Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100, Izmir, Bornova, Turkey. pinar.kara@ege.edu.tr.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2020 Nov; Vol. 412 (28), pp. 7851-7860. Date of Electronic Publication: 2020 Sep 16.
DOI: 10.1007/s00216-020-02916-x
Abstrakt: In the present study, a sensitive electrochemical aptamer-based biosensing strategy for human non-small cell lung cancer (NSCLC) detection was proposed using nanofiber-modified disposable pencil graphite electrodes (PGEs). The composite nanofiber was comprised of polyacrylonitrile (PAN) and polypyrrole (PPy) polymers, and fabrication of the nanofibers was accomplished using electrospinning process onto PGEs. Development of the nanofibers was confirmed using scanning electron microscopy (SEM). The high-affinity 5'-aminohexyl-linked aptamer was immobilized onto a PAN/PPy composite nanofiber-modified sensor surface via covalent bonding strategy. After incubation with NSCLC living cells (A549 cell line) at 37.5 °C, the recognition between aptamer and target cells was monitored by electrochemical impedance spectroscopy (EIS). The selectivity of the aptasensor was evaluated using nonspecific human cervical cancer cells (HeLa) and a nonspecific aptamer sequence. The proposed electrochemical aptasensor showed high sensitivity toward A549 cells with a detection limit of 1.2 × 10 3 cells/mL. The results indicate that our label-free electrochemical aptasensor has great potential in the design of aptasensors for the diagnostics of other types of cancer cells with broad detection capability in clinical analysis. Graphical abstract.
Databáze: MEDLINE
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