Kaposi's sarcoma-associated herpesvirus protein ORF75 among HIV-1 patients in Kenya.
| Autor: | Demba RN; School of Health Sciences, Kisii University, Kisii, Kenya.; Institute of Tropical and Infectious Diseases, University of Nairobi, Nairobi, Kenya., Aradi SM; Department of Internal Medicine, University of Nairobi, Nairobi, Kenya., Mwau M; Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute, Busia, Kenya., Mwanda WO; Institute of Tropical and Infectious Diseases, University of Nairobi, Nairobi, Kenya. |
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| Jazyk: | angličtina |
| Zdroj: | African journal of laboratory medicine [Afr J Lab Med] 2020 Aug 25; Vol. 9 (1), pp. 939. Date of Electronic Publication: 2020 Aug 25 (Print Publication: 2020). |
| DOI: | 10.4102/ajlm.v9i1.939 |
| Abstrakt: | Background: Histology is used to identify Kaposi's sarcoma (KS) in countries with low resources to fund healthcare costs. Approximately 95% of KS cases can be detected using a polymerase chain reaction. Objective: To determine the presence of the open reading frame 75 (ORF75) gene associated with Kaposi's sarcoma herpes virus among HIV-1/AIDS patients and to describe morphological presentations of KS. Methods: This was a retrospective, descriptive study of archived tissue blocks collected from 2013 to 2016. Haematoxylin and eosin staining was used to identify KS. Deoxyribonucleic acid from archived tissue blocks was extracted and a nested polymerase chain reaction was used to detect the ORF75 gene. Results: All 81 cases in this study had been diagnosed as HIV-1 positive, of which 68 had hallmark features of KS in the histology report and 13 had features suggestive of KS ('KS-like'). Microscopic identification of KS by haematoxylin and eosin staining was considered a significant indicator of KS herpes virus ORF75 gene positivity ( p = 0.002). The ORF75 gene was detected in 60.5% (49/81) of tissue blocks; 27.2% were men (22/81) and 33.3% were women (27/81). The ORF75 gene was observed to be present in up to 15.4% (2/13) of the cases reported to have KS-like features. Conclusion: Following the initial diagnosis of KS by histology, the ORF75 gene was fur-ther detected from both cases that had hallmark features of KS as well as among cases with KS-like fea-tures. Competing Interests: The authors declare no conflict of interest. (© 2020. The Authors.) |
| Databáze: | MEDLINE |
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