Gallic and Ellagic Acids Are Promising Adjuvants to Conventional Amphotericin B for the Treatment of Cutaneous Leishmaniasis.

Autor:	Alves MMM; Antileishmanial Activity Laboratory, Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piauí, Brazil mualemmichel@ufpi.edu.br.; Department of Veterinary Morphophysiology, Federal University of Piauí, Teresina, Piauí, Brazil., Arcanjo DDR; Antileishmanial Activity Laboratory, Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piauí, Brazil., Figueiredo KA; Antileishmanial Activity Laboratory, Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piauí, Brazil.; Laboratory of Dermopharmacy, Faculty of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Oliveira JSSM; Laboratory of Natural Products, Department of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil., Viana FJC; Histotechnical Laboratory, Department of Morphology, Federal University of Piauí, Teresina, Piauí, Brazil., Coelho ES; Laboratory of Dermopharmacy, Faculty of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Lopes GLN; Laboratory of Dermopharmacy, Faculty of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Gonçalves JCR; Antileishmanial Activity Laboratory, Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piauí, Brazil.; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil., Carvalho ALM; Laboratory of Dermopharmacy, Faculty of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Rizzo MDS; Histotechnical Laboratory, Department of Morphology, Federal University of Piauí, Teresina, Piauí, Brazil., Chaves MH; Laboratory of Natural Products, Department of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil., Mendonça IL; Department of Veterinary Clinics and Surgery, Federal University of Piauí, Teresina, Piauí, Brazil., Carvalho FAA; Antileishmanial Activity Laboratory, Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piauí, Brazil.
Jazyk:	angličtina
Zdroj:	Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2020 Nov 17; Vol. 64 (12). Date of Electronic Publication: 2020 Nov 17 (Print Publication: 2020).
DOI:	10.1128/AAC.00807-20
Abstrakt:	In this study, we demonstrated the potential associative effect of combining conventional amphotericin B (Amph B) with gallic acid (GA) and with ellagic acid (EA) in topical formulations for the treatment of cutaneous leishmaniasis in BALB/c mice. Preliminary stability tests of the formulations and in vitro drug release studies with Amph B, GA, Amph B plus GA, EA, and Amph B plus EA were carried out, as well as assessment of the in vivo treatment of BALB/c mice infected with Leishmania major After 40 days of infection, the animals were divided into 6 groups and treated twice a day for 21 days with a gel containing Amph B, GA, Amph B plus GA, EA, or Amph B plus EA, and the negative-control group was treated with the vehicle. In the animals that received treatment, there was reduction of the lesion size and reduction of the parasitic load. Histopathological analysis of the treatments with GA, EA, and combinations with Amph B showed circumscribed lesions with the presence of fibroblasts, granulation tissue, and collagen deposition, as well as the presence of activated macrophages. The formulations containing GA and EA activated macrophages in all evaluated parameters, resulting in the activation of cells of the innate immune response, which can generate healing and protection. GA and EA produced an associative effect with Amph B, which makes them promising for use with conventional Amph B in the treatment of cutaneous leishmaniasis. (Copyright © 2020 American Society for Microbiology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu