Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats.

Autor:	Somade OT; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria., Ajayi BO; Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Nigeria., Adeyi OE; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria., Adeshina AA; School of Medicine, All Saint University, Roseau, Dominica., James AS; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria., Ayodele PF; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria.
Jazyk:	angličtina
Zdroj:	Metabolism open [Metabol Open] 2020 Aug 17; Vol. 7, pp. 100051. Date of Electronic Publication: 2020 Aug 17 (Print Publication: 2020).
DOI:	10.1016/j.metop.2020.100051
Abstrakt:	Ethylene glycol monomethyl ether (EGME) is a major component of paints, lacquers, inks, and automobile brake fluids. As a result, exposures to humans are inevitable. We therefore, investigated in this study, its effect on testicular cells in a time-course manner in male Wistar rats. Animals were orally administered 50 mg/kg body weight of EGME for duration of 7, 14, and 21 days. Following 7 days of the administration, levels of NO and GSH were significantly reduced, while levels of c-Myc, K-Ras, caspase-3, IL-6, TNF-α, and IL-1β were significantly increased compared with control. At the end of 14 days exposure, GPx, and SOD activities, as well as IL-10 level were significantly decreased, while levels of c-Myc, K-Ras, p53, Bax, caspase-3, IL-6, TNF-α, IL-1β, and GST activity were significantly elevated compared with control. After 21 days of EGME administration, Bcl-2, IL-10, and NO levels were significantly decreased, while levels of c-Myc, K-Ras, p53, Bax, caspase-3, IL-6, TNF-α, IL-1β, MDA and GST activity were significantly increased compared with control. After 7, 14, and 21 days of EGME administrations, testis histopathology showed severe loss of seminiferous tubules, the seminiferous epithelium revealed very few spermatocytes, spermatids, spermatogonia, spermatozoa, and Sertoli cells, while the interstitial tissue is eroded, with scanty abnormal Leydig cells, compared with the control that appeared normal. We therefore, concluded that EGME-induced testicular toxicity as a result of EGME administration could be via the disorganization of the endogenous antioxidant systems as well as up-regulation of pro-inflammatory, apoptotic and oncogenic mediators in rats. Competing Interests: None to declare. (© 2020 The Authors.)
Databáze:	MEDLINE
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