Cardiolipin, conformation, and respiratory complex-dependent oligomerization of the major mitochondrial ADP/ATP carrier in yeast.

Autor:	Senoo N; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Kandasamy S; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Ogunbona OB; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Baile MG; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Lu Y; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Claypool SM; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 Aug 28; Vol. 6 (35), pp. eabb0780. Date of Electronic Publication: 2020 Aug 28 (Print Publication: 2020).
DOI:	10.1126/sciadv.abb0780
Abstrakt:	The phospholipid cardiolipin has pleiotropic structural and functional roles that are collectively essential for mitochondrial biology. Yet, the molecular details of how this lipid supports the structure and function of proteins and protein complexes are poorly understood. To address this property of cardiolipin, we use the mitochondrial adenosine 5'-diphosphate/adenosine 5'-triphosphate carrier (Aac) as a model. Here, we have determined that cardiolipin is critical for both the tertiary and quaternary assembly of the major yeast Aac isoform Aac2 as well as its conformation. Notably, these cardiolipin-provided structural roles are separable. In addition, we show that multiple copies of Aac2 engage in shared complexes that are largely dependent on the presence of assembled respiratory complexes III and IV or respiratory supercomplexes. Intriguingly, the assembly state of Aac2 is sensitive to its transport-related conformation. Together, these results expand our understanding of the numerous structural roles provided by cardiolipin for mitochondrial membrane proteins. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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