SARS-CoV-2 seroprevalence and asymptomatic viral carriage in healthcare workers: a cross-sectional study.

Autor:	Shields A; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK.; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Faustini SE; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Perez-Toledo M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK., Jossi S; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK., Aldera E; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK., Allen JD; School of Biological Sciences, University of Southampton, Southampton, UK., Al-Taei S; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Backhouse C; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Bosworth A; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Dunbar LA; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Ebanks D; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Emmanuel B; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Garvey M; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK., Gray J; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Kidd IM; Public Health England Midlands and East Region, Birmingham, UK., McGinnell G; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., McLoughlin DE; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Morley G; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., O'Neill J; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK., Papakonstantinou D; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK., Pickles O; Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK., Poxon C; Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK., Richter M; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK., Walker EM; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK., Wanigasooriya K; Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK., Watanabe Y; School of Biological Sciences, University of Southampton, Southampton, UK.; Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, UK., Whalley C; Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK., Zielinska AE; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK., Crispin M; School of Biological Sciences, University of Southampton, Southampton, UK., Wraith DC; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.; University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, NIHR Biomedical Research Centre, Birmingham, UK., Beggs AD; Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK., Cunningham AF; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK., Drayson MT; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK.; University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, NIHR Biomedical Research Centre, Birmingham, UK., Richter AG; Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK a.g.richter@bham.ac.uk.; University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
Jazyk:	angličtina
Zdroj:	Thorax [Thorax] 2020 Dec; Vol. 75 (12), pp. 1089-1094. Date of Electronic Publication: 2020 Sep 11.
DOI:	10.1136/thoraxjnl-2020-215414
Abstrakt:	Objective: To determine the rates of asymptomatic viral carriage and seroprevalence of SARS-CoV-2 antibodies in healthcare workers. Design: A cross-sectional study of asymptomatic healthcare workers undertaken on 24/25 April 2020. Setting: University Hospitals Birmingham NHS Foundation Trust (UHBFT), UK. Participants: 545 asymptomatic healthcare workers were recruited while at work. Participants were invited to participate via the UHBFT social media. Exclusion criteria included current symptoms consistent with COVID-19. No potential participants were excluded. Intervention: Participants volunteered a nasopharyngeal swab and a venous blood sample that were tested for SARS-CoV-2 RNA and anti-SARS-CoV-2 spike glycoprotein antibodies, respectively. Results were interpreted in the context of prior illnesses and the hospital departments in which participants worked. Main Outcome Measure: Proportion of participants demonstrating infection and positive SARS-CoV-2 serology. Results: The point prevalence of SARS-CoV-2 viral carriage was 2.4% (n=13/545). The overall seroprevalence of SARS-CoV-2 antibodies was 24.4% (n=126/516). Participants who reported prior symptomatic illness had higher seroprevalence (37.5% vs 17.1%, χ 2 =21.1034, p<0.0001) and quantitatively greater antibody responses than those who had remained asymptomatic. Seroprevalence was greatest among those working in housekeeping (34.5%), acute medicine (33.3%) and general internal medicine (30.3%), with lower rates observed in participants working in intensive care (14.8%). BAME (Black, Asian and minority ethnic) ethnicity was associated with a significantly increased risk of seropositivity (OR: 1.92, 95% CI 1.14 to 3.23, p=0.01). Working on the intensive care unit was associated with a significantly lower risk of seropositivity compared with working in other areas of the hospital (OR: 0.28, 95% CI 0.09 to 0.78, p=0.02). Conclusions and Relevance: We identify differences in the occupational risk of exposure to SARS-CoV-2 between hospital departments and confirm asymptomatic seroconversion occurs in healthcare workers. Further investigation of these observations is required to inform future infection control and occupational health practices. Competing Interests: Competing interests: MTD reports personal fees from Abingdon Health, outside the submitted work. All other authors declare no competing interests. (© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze:	MEDLINE
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