The optoretinogram reveals the primary steps of phototransduction in the living human eye.

Autor:	Pandiyan VP; Department of Ophthalmology, University of Washington, Seattle, WA 98109, USA., Maloney-Bertelli A; Department of Ophthalmology, University of Washington, Seattle, WA 98109, USA., Kuchenbecker JA; Department of Ophthalmology, University of Washington, Seattle, WA 98109, USA., Boyle KC; Hansen Experimental Physics Laboratory, Stanford, CA 94305, USA.; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA., Ling T; Hansen Experimental Physics Laboratory, Stanford, CA 94305, USA.; Department of Ophthalmology, Stanford University, Stanford, CA 94305, USA., Chen ZC; Hansen Experimental Physics Laboratory, Stanford, CA 94305, USA.; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA., Park BH; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Roorda A; School of Optometry, University of California, Berkeley, CA 94720, USA., Palanker D; Hansen Experimental Physics Laboratory, Stanford, CA 94305, USA.; Department of Ophthalmology, Stanford University, Stanford, CA 94305, USA., Sabesan R; Department of Ophthalmology, University of Washington, Seattle, WA 98109, USA. rsabesan@uw.edu.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 Sep 09; Vol. 6 (37). Date of Electronic Publication: 2020 Sep 09 (Print Publication: 2020).
DOI:	10.1126/sciadv.abc1124
Abstrakt:	Photoreceptors initiate vision by converting photons to electrical activity. The onset of the phototransduction cascade is marked by the isomerization of photopigments upon light capture. We revealed that the onset of phototransduction is accompanied by a rapid (<5 ms), nanometer-scale electromechanical deformation in individual human cone photoreceptors. Characterizing this biophysical phenomenon associated with phototransduction in vivo was enabled by high-speed phase-resolved optical coherence tomography in a line-field configuration that allowed sufficient spatiotemporal resolution to visualize the nanometer/millisecond-scale light-induced shape change in photoreceptors. The deformation was explained as the optical manifestation of electrical activity, caused due to rapid charge displacement following isomerization, resulting in changes of electrical potential and surface tension within the photoreceptor disc membranes. These all-optical recordings of light-induced activity in the human retina constitute an optoretinogram and hold remarkable potential to reveal the biophysical correlates of neural activity in health and disease. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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