Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children.

Autor: Lima R; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA.; Department of Pediatrics, Division of Gastroenterology and Nutrition, Massachusetts General Hospital, Boston, MA, USA., Gootkind EF; Department of Pediatrics, Pulmonary Division, Massachusetts General Hospital, Boston, MA, USA., De la Flor D; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA., Yockey LJ; Department of Internal Medicine, Massachusetts General Hospital, Boston, MA, USA.; Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA., Bordt EA; Department of Pediatrics, Lurie Center for Autism, Massachusetts General Hospital, Boston, MA, USA., D'Avino P; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA., Ning S; Department of Neurology, Massachusetts General Hospital, Boston, MA, USA., Heath K; Department of Dermatology, Cutaneous Biology Research Center, Massachusetts General Hospital, Boston, MA, USA., Harding K; Department of Dermatology, Cutaneous Biology Research Center, Massachusetts General Hospital, Boston, MA, USA., Zois J; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA., Park G; Department of Pediatrics, Pulmonary Division, Massachusetts General Hospital, Boston, MA, USA., Hardcastle M; Department of Pediatrics, Pulmonary Division, Massachusetts General Hospital, Boston, MA, USA., Grinke KA; Translational and Clinical Research Center, Massachusetts General Hospital, Boston, MA, USA., Grimmel S; Translational and Clinical Research Center, Massachusetts General Hospital, Boston, MA, USA., Davidson SP; Translational and Clinical Research Center, Massachusetts General Hospital, Boston, MA, USA., Forde PJ; Translational and Clinical Research Center, Massachusetts General Hospital, Boston, MA, USA., Hall KE; Translational and Clinical Research Center, Massachusetts General Hospital, Boston, MA, USA., Neilan AM; Department of Internal Medicine, Massachusetts General Hospital, Boston, MA, USA.; Department of Pediatrics, Infectious Disease, Massachusetts General Hospital, Boston, MA, USA., Matute JD; Department of Pediatrics, Division of Neonatology and Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Lerou PH; Department of Pediatrics, Division of Neonatology and Newborn Medicine, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Fasano A; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA.; Department of Pediatrics, Division of Gastroenterology and Nutrition, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Shui JE; Department of Pediatrics, Infectious Disease, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Edlow AG; Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Yonker LM; Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, USA. lyonker@mgh.harvard.edu.; Department of Pediatrics, Pulmonary Division, Massachusetts General Hospital, Boston, MA, USA. lyonker@mgh.harvard.edu.; Harvard Medical School, Boston, MA, USA. lyonker@mgh.harvard.edu.
Jazyk: angličtina
Zdroj: BMC medical research methodology [BMC Med Res Methodol] 2020 Sep 11; Vol. 20 (1), pp. 228. Date of Electronic Publication: 2020 Sep 11.
DOI: 10.1186/s12874-020-01110-y
Abstrakt: Background: COVID-19, the disease caused by the highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although the impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, and adolescents in a standardized manner during the COVID-19 pandemic is essential to establish a biologic understanding of the disease in the pediatric population. This biorepository enables pediatric centers world-wide to collect samples uniformly to drive forward our understanding of COVID-19 by addressing specific pediatric and neonatal COVID-19-related questions.
Methods: A COVID-19 biospecimen collection study was implemented with strategic enrollment guidelines to include patients seen in urgent care clinics and hospital settings, neonates born to SARS-CoV-2 infected mothers, and asymptomatic children. The methodology described here, details the importance of establishing collaborations between the clinical and research teams to harmonize protocols for patient recruitment and sample collection, processing and storage. It also details modifications required for biobanking during a surge of the COVID-19 pandemic.
Results: Considerations and challenges facing enrollment of neonatal and pediatric cohorts are described. A roadmap is laid out for successful collection, processing, storage and database management of multiple pediatric samples such as blood, nasopharyngeal and oropharyngeal swabs, sputum, saliva, tracheal aspirates, stool, and urine. Using this methodology, we enrolled 327 participants, who provided a total of 972 biospecimens.
Conclusions: Pediatric biospecimens will be key in answering questions relating to viral transmission by children, differences between pediatric and adult viral susceptibility and immune responses, the impact of maternal SARS-CoV-2 infection on fetal development, and factors driving the Multisystem Inflammatory Syndrome in Children. The specimens in this biorepository will allow necessary comparative studies between children and adults, help determine the accuracy of current pediatric viral testing techniques, in addition to, understanding neonatal exposure to SARS-CoV-2 infection and disease abnormalities. The successful establishment of a pediatric biorepository is critical to provide insight into disease pathogenesis, and subsequently, develop future treatment and vaccination strategies.
Databáze: MEDLINE
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