Anti-biofilm and wound-healing activity of chitosan-immobilized Ficin.

Autor: Baidamshina DR; Kazan (Volga Region) Federal University, Kazan, Russia., Koroleva VA; Voronezh State University, Voronezh, Russia., Trizna EY; Kazan (Volga Region) Federal University, Kazan, Russia., Pankova SM; Voronezh State University, Voronezh, Russia., Agafonova MN; Kazan (Volga Region) Federal University, Kazan, Russia., Chirkova MN; Kazan (Volga Region) Federal University, Kazan, Russia., Vasileva OS; Kazan (Volga Region) Federal University, Kazan, Russia., Akhmetov N; Kazan (Volga Region) Federal University, Kazan, Russia., Shubina VV; Kazan (Volga Region) Federal University, Kazan, Russia., Porfiryev AG; Kazan (Volga Region) Federal University, Kazan, Russia., Semenova EV; Kazan (Volga Region) Federal University, Kazan, Russia., Sachenkov OA; Kazan (Volga Region) Federal University, Kazan, Russia., Bogachev MI; St Petersburg Electrotechnical University, St. Petersburg, Russia., Artyukhov VG; Voronezh State University, Voronezh, Russia., Baltina TV; Kazan (Volga Region) Federal University, Kazan, Russia., Holyavka MG; Voronezh State University, Voronezh, Russia., Kayumov AR; Kazan (Volga Region) Federal University, Kazan, Russia. Electronic address: kairatr@yandex.ru.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 01; Vol. 164, pp. 4205-4217. Date of Electronic Publication: 2020 Sep 08.
DOI: 10.1016/j.ijbiomac.2020.09.030
Abstrakt: Biofouling is among the key factors slowing down healing of acute and chronic wounds. Here we report both anti-biofilm and wound-healing properties of the chitosan-immobilized Ficin. The proposed chitosan-adsorption approach allowed preserving ~90% of the initial total activity of the enzyme (when using azocasein as a substrate) with stabilization factor of 4.9, and ~70% of its specific enzymatic activity. In vitro, the chitosan-immobilized Ficin degraded staphylococcal biofilms, this way increasing the efficacy of antimicrobials against biofilm-embedded bacteria. In vivo, in the presence of Ficin (either soluble or immobilized), the S.aureus-infected skin wound areas in rats reduced twofold after 4 instead of 6 days treatment. Moreover, topical application of the immobilized enzyme resulted in a 3-log reduction of S. aureus cell count on the wound surfaces in 6 days, compared to more than 10 days required to achieve the same effect in control. Additional advantages include smoother reepithelisation, and new tissue formation exhibiting collagen structure characteristics closely reminiscent of those observed in the native tissue. Taken together, our data suggest that both soluble and immobilized Ficin appear beneficial for the treatment of biofilm-associated infections, as well as speeding up wound healing and microbial decontamination.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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