Biochemical characterization and in vitro biological activities of the epithelial cell extracts from Hypanus dipterurus spine.

Autor: Marrón-Grijalba E; Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, La Paz, Baja California Sur, 23096, Mexico. Electronic address: estrelladel.marrongri@hotmail.com., Cardona-Félix CS; CONACyT-Instituto Politécnico Nacional-CICIMAR, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, La Paz, Baja California Sur, 23096, Mexico. Electronic address: cscardonafe@conacyt.mx., Cruz-Escalona VH; Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, La Paz, Baja California Sur, 23096, Mexico. Electronic address: vescalon@ipn.mx., Muñoz-Ochoa M; Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, La Paz, Baja California Sur, 23096, Mexico. Electronic address: mmunozo@ipn.mx., Cabral-Romero C; Universidad Autónoma de Nuevo León, UANL, Facultad de Odontología, Laboratorio de Biología Molecular, Dr. Eduardo Aguirre Pequeño, Mitras Centro, 64460, Monterrey, N.L., Mexico. Electronic address: claudio.cabralrm@uanl.edu.mx., Hernández-Delgadillo R; Universidad Autónoma de Nuevo León, UANL, Facultad de Odontología, Laboratorio de Biología Molecular, Dr. Eduardo Aguirre Pequeño, Mitras Centro, 64460, Monterrey, N.L., Mexico. Electronic address: rene.hernandezdl@uanl.edu.mx., Rivera-Pérez C; Centro de Investigaciones Biológicas Del Noroeste, Km. 1 Carretera a San Juan de La Costa, La Paz, BCS, 23205, Mexico. Electronic address: crivera@cibnor.mx., Aguila-Ramírez RN; Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, La Paz, Baja California Sur, 23096, Mexico. Electronic address: raguilar@ipn.mx.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Nov; Vol. 187, pp. 129-135. Date of Electronic Publication: 2020 Sep 08.
DOI: 10.1016/j.toxicon.2020.08.032
Abstrakt: Ocean organisms live in competitive environments that demand the production of poisons and toxins. In some cases, these substances have been used in the pharmaceutical industry for human disease treatments. Most fish poisons generally have potent cytolytic activity, probably through cardiovascular and neuromuscular effects. In the case of marine stingrays, the injuries made by their tail venom apparatus are caused by the mechanical penetration of their sting and a subsequent venom release. This study focused on the evaluation of substances with cytotoxic activity in the epithelium that covers the venom apparatus from the marine stingray Hypanus dipterurus. To demonstrate the above, the hemolytic, proteolytic and cytotoxic capacities of H. dipterurus epithelium substances were determined. Discs impregnated with epithelial extract were used on blood agar plates. The proteolytic activity was analyzed using casein as substrate and for gelatin the liquefaction activity test. To determine the cytotoxicity degree of the extracts, the proliferation and cell viability MTT bioassay was implemented on human cervical carcinoma cells (HeLa). The results showed that no hemolytic or proteolytic activity existed against casein associated with the epithelial extract, but gelatin hydrolysis and cytotoxic activity against the HeLa cell line were observed. This study concludes that the substances found in the epithelium covering the H. dipterurus stingray venom apparatus are a mixture of various proteins, among which, glycosylated anionic proteins represent a potential source of molecules with cytotoxic and hydrolytic activity.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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