Synthesis and characterization of V 2 O 5 /urease for a biosensor of urea.

Autor: da Rocha RCF; Departamento de Química e Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei-CAP, Ouro Branco, MG, Brazil., de Souza FA; Departamento de Química e Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei-CAP, Ouro Branco, MG, Brazil., Vieira NS; Departamento de Química e Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei-CAP, Ouro Branco, MG, Brazil., Cestarolli DT; Departamento de Química e Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei-CAP, Ouro Branco, MG, Brazil., Guerra EM; Departamento de Química e Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei-CAP, Ouro Branco, MG, Brazil.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2021 Oct; Vol. 68 (5), pp. 1027-1032. Date of Electronic Publication: 2020 Sep 24.
DOI: 10.1002/bab.2022
Abstrakt: Vanadium pentoxide (V 2 O 5 ) was used as support material to immobilize the urease enzyme. V 2 O 5 was prepared from a sol-gel process, and the immobilization of urease was done onto the surface of the PET/ITO/V 2 O 5 film. Different concentrations of urea were tested to study the sensitivity of the biosensor. Tests with pH variation were carried out, and it was noticed that the total charge in the cyclic voltammograms decreases due to the increase in pH that influences the catalytic activity of the enzyme. From cyclic voltammograms, with a different scan rate, for linear correlation testing, it was observed that the anodic currents varied as a function of the scan rate. Based on the variation in the urea concentration, the current increased with the increase in the urea concentration, indicating the sensitivity of the material. The use of V 2 O 5 /urease showed favorable electrochemical responses for application in biosensors as well as being sensitive to the variation in the urea concentration and easy to obtain and prepare.
(© 2020 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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