Cardiovascular and body weight regulation changes in transgenic mice overexpressing thyrotropin-releasing hormone (TRH).

Autor: Landa MS; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina. mslanda@conicet.gov.ar.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Department of Molecular Genetics and Biology of Complex Diseases, Universidad de Buenos Aires, Buenos Aires, Argentina. mslanda@conicet.gov.ar., García SI; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Laboratory of Molecular Cardiology, Universidad de Buenos Aires, Buenos Aires, Argentina.; Laboratorio de Medicina Experimental, Hospital Aleman, Buenos Aires, Argentina., Schuman ML; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Laboratory of Molecular Cardiology, Universidad de Buenos Aires, Buenos Aires, Argentina., Peres Diaz LS; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Laboratory of Molecular Cardiology, Universidad de Buenos Aires, Buenos Aires, Argentina., Aisicovich M; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Laboratory of Molecular Cardiology, Universidad de Buenos Aires, Buenos Aires, Argentina., Pirola CJ; School of Medicine, Institute of Medical Research A Lanari, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina. pirola.carlos@lanari.uba.ar.; National Scientific and Technical Research Council (CONICET), Institute of Medical Research (IDIM), Department of Molecular Genetics and Biology of Complex Diseases, Universidad de Buenos Aires, Buenos Aires, Argentina. pirola.carlos@lanari.uba.ar.
Jazyk: angličtina
Zdroj: Journal of physiology and biochemistry [J Physiol Biochem] 2020 Nov; Vol. 76 (4), pp. 599-608. Date of Electronic Publication: 2020 Sep 11.
DOI: 10.1007/s13105-020-00765-x
Abstrakt: Thyrotropin-releasing hormone (TRH) plays several roles as a hormone/neuropeptide. Diencephalic TRH (dTRH) participates in the regulation of blood pressure in diverse animal models, independently of the thyroid status. The present study aimed to evaluate whether chronic overexpression of TRH in mice affects cardiovascular and metabolic variables. We developed a transgenic (TG) mouse model that overexpresses dTrh. Despite having higher food consumption and water intake, TG mice showed significantly lower body weight respect to controls. Also, TG mice presented higher blood pressure, heart rate, and locomotor activity independently of thyroid hormone levels. These results and the higher urine noradrenaline excretion observed in TG mice suggest a higher metabolic rate mediated by sympathetic overflow. Cardiovascular changes were impeded by siRNA inhibition of the diencephalic Trh overexpression. Also, the silencing of dTRH in the TG mice normalized urine noradrenaline excretion, supporting the view that the cardiovascular effects of TRH involve the sympathetic system. Overall, we show that congenital dTrh overexpression leads to an increase in blood pressure accompanied by changes in body weight and food consumption mediated by a higher sympathetic overflow. These results provide new evidence confirming the participation of TRH in cardiovascular and body weight regulation.
Databáze: MEDLINE
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