Lactation in large litters influences anxiety, memory, and spreading depression in adult male rats that were chronically subjected to a non-convulsive pilocarpine dose.

Autor: Benevides RDL; Department of Nutrition, Universidade Federal de Pernambuco, Recife, PE, Brazil., de-Lima SMV; Department of Nutrition, Universidade Federal de Pernambuco, Recife, PE, Brazil., Chagas CL; Department of Nutrition, Universidade Federal de Pernambuco, Recife, PE, Brazil., de Lima CF; Department of Nutrition, Universidade Federal de Pernambuco, Recife, PE, Brazil., Abadie-Guedes R; Department of Physiology and Pharmacology, Universidade Federal de Pernambuco, Recife, PE, Brazil., Guedes RCA; Department of Nutrition, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Jazyk: angličtina
Zdroj: Nutritional neuroscience [Nutr Neurosci] 2022 Apr; Vol. 25 (4), pp. 846-856. Date of Electronic Publication: 2020 Sep 11.
DOI: 10.1080/1028415X.2020.1819103
Abstrakt: Objectives: Unfavorable lactation influences brain excitability and behavioral reactions in adults. Administration early in life of the cholinergic agonist, pilocarpine, even at non-convulsive doses, alters the brain excitability-related phenomenon known as cortical spreading depression (CSD), and produce anxiogenic-like behavior. However, the influence of unfavorable lactation on the CSD- and memory-effects of pilocarpine administration late in life has not been investigated. Herein, we analyzed the ponderal, electrophysiological (CSD), and behavioral effects of chronic treatment with a non-convulsive dose of pilocarpine, in adult rats suckled under favorable and unfavorable conditions. Methods: Wistar rats were suckled in litters with 9 or 15 pups (groups L 9 and L 15 , respectively). A very low dose of pilocarpine (45/mg/kg/day) was chronically administered in mature rats from postnatal day (PND) 69-90. Behavioral tests occurred at PND91 [elevated plus maze (EPM)], PND93 [open field (OF)], and PND94-95 [object recognition memory (ORM)]. CSD was recorded between PND96-120. Results: Pilocarpine-treated rats performed worse in the anxiety and memory tests, and displayed lower CSD propagation velocity when compared with saline-treated controls. In addition, L 15 rats showed an increase in the distance traveled and a decrease in the immobility time in the EPM, impaired ORM, and accelerated CSD propagation when compared with L 9 rats ( p  ≤ 0.05). Discussion: These data suggest that sub-convulsive pilocarpine treatment in adult rats can affect behavioral and excitability-related reactions. In addition, unfavorable lactation increases the ambulatory effects of pilocarpine. Further studies should investigate the possible cholinergic molecular mechanisms involved in these effects.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje