| Autor: |
Gálvez-Iriqui AC; Departamento de Investigación y Posgrado en Alimentos, Microbiology and Mycotoxins Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México., García-Romo JS; Departamento de Investigación y Posgrado en Alimentos, Microbiology and Mycotoxins Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México., Cortez-Rocha MO; Departamento de Investigación y Posgrado en Alimentos, Microbiology and Mycotoxins Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México., Burgos-Hernández A; Departamento de Investigación y Posgrado en Alimentos, Microbiology and Mycotoxins Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México., Burboa-Zazueta MG; Departamento de Investigaciones Científicas y Tecnológicas, Cell Biology Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México., Luque-Alcaraz AG; Departamento de Ingeniería Biomédica, Universidad Estatal de Sonora, Ley Federal del Trabajo S/N, Col. Apolo, 83100, Hermosillo, Sonora, México., Calderón-Santoyo M; Integral Laboratory of Food Research, Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, 63175, Tepic, Nayarit, México., Argüelles-Monal WM; Biopolymer Laboratory, Centro de Investigación y Desarrollo en Alimentación, A.C., Carretera Gustavo Enrique Astiazarán Rosas, N0. 46, 83304, Hermosillo, Sonora, Mexico., Plascencia-Jatomea M; Departamento de Investigación y Posgrado en Alimentos, Microbiology and Mycotoxins Laboratory, Blvd. Luis Encinas y Rosales S/N, Col. Centro, 83000, Hermosillo, Sonora, México. maribel.plascencia@unison.mx. |
| Abstrakt: |
Chitosan (CS) nanosystems have potential applications for the control of microorganisms in the medical, environmental, and agrifood fields. In vivo and in vitro assays of CS nanosystems have experienced increased activity due to improved physicochemical properties, biological activity, and reactivity. Hence, it is important to determine whether their application involves toxicological risks. The aim of this study was to evaluate the mutagenic, cytotoxic, phytotoxic, and in vivo antifungal activity of chitosan-pyrrole-2-carboxylic acid nanobiocomposites (CS-PCA). The CS-PCA nanoparticles were synthesized by means of the nanoprecipitation technique with a size and ζ-potential of 502 ± 72 nm and + 54.7 ± 15.0 mV, respectively. According to the Ames test, no evidence of mutagenic activity was observed in Salmonella typhimurium strains. The cytotoxic assay showed that the incorporation of PCA into the CS matrix increased the toxic effect on ARPE-19 cells. However, fluorescence microscopy of ARPE-19 cells did not reveal morphostructural changes allusive to cell injury. CS-PCA exhibited strong phytotoxicity on lettuce seeds and the complete inhibition of seed development. The antifungal assay demonstrated that the CS-PCA delayed Aspergillus niger infection in tomato fruit until day 3; however, its use for the pre-treatment of seeds might exert adverse effects on plant development. |
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